口腔疾病防治2017，Vol.25Issue(1)：21-25,5.DOI:10.12016/j.issn.2096-1456.2017.01.004
槟榔碱和Ca2+对口腔黏膜异常增生上皮体外模型通透性的影响
Effects of arecoline and calcium ion on the permeability of dysplastic oral epithelia model in vitro
摘要
Abstract
Objective To study the effects of arecolineand Calcium ion (Ca2+) on the permeability of dysplastic oral epithelia model in vitro. Methods To establish the dysplastic oral epithelia model in vitro by culturing oral keratinocyte (DOK) and harvesting a DOK cell monolayer. The models were divided into control group, arecoline group and"Ca 2++are⁃coline"group. The values of Lucifer Yellow Papp were used to estimate the permeability changes of models after treated⁃with arecoline and Ca2+. Results In arecoline group, the lucifer yellow Papp values of each subgroup were higher than that of control group (P < 0.05), and the values increased as arecoline concentration elevated and time lasted (P < 0.05). When the"Ca2++arecoline"group were pretreated with Ca2+, the values of subgroup"10μg/mL"was lower than that of the corresponding arecoline group (P>0.05). However, the value of subgroup"4 h 10μg/mL"of"Ca2++arecoline"group had no statistical difference with that of control group (P>0.05), while the other subgroups were increased (P<0.05);Be⁃sides, these values in"Ca2++arecoline"group were increased as the arecoline concentration elevated and time lasted too (P<0.05). Conclusion Intervention of arecoline contributes to the increase of permeability of DOK cell monolayer mod⁃el, which maybe an important reason for the cancerization of dysplastic oral epithelia, however Ca2+might weaken these ef⁃fects of arecoline in the process.关键词
槟榔碱/口腔黏膜异常增生上皮/体外模型/Ca2+/通透性Key words
Arecoline/Dysplastic oral epithelia/In vitro model/Calcium/Permeability分类
医药卫生引用本文复制引用
侯冬兰,陈蓉,高义军,尹晓敏..槟榔碱和Ca2+对口腔黏膜异常增生上皮体外模型通透性的影响[J].口腔疾病防治,2017,25(1):21-25,5.基金项目
湖南省自然科学基金资助项目（14JJ2030) （）
