口腔疾病防治2017，Vol.25Issue(1)：26-30,5.DOI:10.12016/j.issn.2096-1456.2017.01.005
高糖对人骨髓间充质干细胞成骨分化的影响
Effects of high glucose on osteogenic differentiation of hBMSC
摘要
Abstract
Objective To investigate the effects of different glucose concentration on the proliferation and osteogen⁃ic differentiation of human bone marrow mesenchymal stem cells (hBMSC) in vivo. Methods Cultured with basal medi⁃um containing different glucose concentrations, CCK⁃8 cell proliferation was detected at 1, 4, 7, 10 days. The osteogenic differentiation of human bone marrow mesenchymal stem cells was observed at 7 d, which was induced by osteogenic differentiation medium with different concentration of glucose. The expressions of alkaline phosphatase (ALP), osteocal⁃cin (OC) and collagen type I (Col⁃1) gene were detected by real⁃time fluorescence quantitative PCR. Mineralized nodule formation was displayed by calciumalizarin red staining on the seventh day. Results 10 mM glucose stimulated prolif⁃eration of hBMSC, while the higher (>30 mM) inhibited the proliferation (P < 0.05); Osteogenic induction can induce osteogenic differentiation of hBMSC, but the increase of glucose concentration will decrease the formation of mineralized nodules of hBMSC, inhibit the expression of osteogenic marker genes ALP, OC and Col⁃1 (P<0.05). Conclusion The expression of Col⁃1, ALP and OC in osteoblast was down⁃regulated by high glucose, and the hBMSC proliferation was in⁃hibited. At the same time, high glucose can reduce the osteogenic mineralization ability of stem cells and indirectly af⁃fect bone formation and metabolism.关键词
糖尿病/骨结合/种植体/成骨分化/人骨髓间充质干细胞Key words
Diabetes mellitus/Osteointegration/Dental implants/Osteogenic differentiation/hBMSC分类
医药卫生引用本文复制引用
高海,陈潇,管东华,张颖婕,林常绿..高糖对人骨髓间充质干细胞成骨分化的影响[J].口腔疾病防治,2017,25(1):26-30,5.基金项目
广东省科技计划项目 （）
