广东医学2016，Vol.37Issue(23)：3485-3489,5.
垂体腺苷酸环化酶激活肽及其衍生物对小鼠胸腺微环境内功能细胞的影响
The influence of neuropeptide PACAP and recombinant PACAP-PTD containing transduction domain on thy-mus microenvironment function cell of mice
摘要
Abstract
Objective To study the protective effects of pituitary adenylate cyclase activating polypeptide ( pitui-tary adenylate cyclase activating polypeptide , PACAP) and its derivative peptide on the thymus microenvironment func-tions cell injury induced by dexamethasone .Methods The primary cells were processed by dexamethasone (Dex).The proliferation and cytoxicity assay kit (CCK8) were applied for detecting the cell activity of processed cells .Annexin V-FITC/PI double staining flow cytometry was applied for detecting the cell proptosis .ELISA was applied to measure the ex-pression of cytokines in co -culture system.Results The activity of processed cells was significantly reduced .Flow cy-tometry showed significantly increase survival and reduction in apoptosis in PACAP -PTD and PACAP groups ( P <0.05).ELISA results showed that the content of cytokines in dosing group were also significantly improved .Conclusion PACAP-PTD and PACAP protect the on the function cells of thymus microenvironment from apoptosis induced by dex -amethasone , and PACAP-PTD is more apparent than PACAP .关键词
胸腺微环境/地塞米松/PACAP-PTD/垂体腺苷酸环化酶激活肽Key words
thymus microenvironment/Dexamethasone/PACAP/PACAP-PTD引用本文复制引用
梁阳,郑丹丹,欧阳军,余榕捷,张华华..垂体腺苷酸环化酶激活肽及其衍生物对小鼠胸腺微环境内功能细胞的影响[J].广东医学,2016,37(23):3485-3489,5.基金项目
国家自然科学基金资助项目(编号:31200679),广东省科技计划项目(编号:2011B031800388),广东省大学生创新训练项目(编号:1057112027) （编号:31200679）
