广东医学2016，Vol.37Issue(24)：3645-3649,5.
高浓度氢气吸入对大鼠局灶性脑缺血再灌注损伤脑皮质神经细胞凋亡及凋亡相关蛋白表达的影响
Effects of inhaling high concentrations hydrogen gas on neuronal apoptosis and the expression of apoptosis related proteins in the cortical region of rats with cerebral ischemia-reperfusion injury
摘要
Abstract
Objective To investigate the effects of inhalation of high concentrations hydrogen gas on neuronal ap -optosis and the expressions of apoptosis -related proteins Bcl -2 and Bax on focal cerebral ischemia reperfusion injury in rats.Methods Forty-eight healthy male SD rats were randomly divided into 3 groups (n=16), sham-operated group (sham group), ischemia-reperfusion group (I/R group, during reperfusion inhaled 67%N2 +33%O2) and inhalation of hydrogen group (H2 group, during reperfusion inhaled 67%H2 +33%O2).Middle cerebral artery occlusion (MCAO) model was constructed using conventional method .Neurological severity score was evaluated 24 h after cerebral reperfusion in all groups .The cerebral infarction severity and volume were assessed by TTC staining , and neuronal apoptosis of brain cortex was examined by Nissl stain and TUNEL technique .The expressions of Bcl -2 and Bax were assessed with Western blot.Results As compared with sham group , the neurological deficit score , brain infarction volume , apoptosis index (AI) and the expression of Bax in I/R group and H2 group were significantly increased (P<0.05).Compared with I/R group, the neurological deficit score , brain infarction volume , apoptosis index and the expression of Bax in H 2 group were markedly decreased , while the expression of Bcl -2 was dramatically increased , and so was the quantity of Nissel′s body (P<0.05).Conclusion High concentration hydrogen during reperfusion can protect the I /R rats by reducing the size of cerebral infarction , ameliorating the neurological deficit and neuron apoptosis , and which may be related with up -regula-ting Bcl-2 and down-regulating Bax .关键词
氢气/脑缺血再灌注/细胞凋亡/凋亡相关蛋白Key words
hydrogen/cerebral ischemia-reperfusion/apoptosis/apoptosis-related protein引用本文复制引用
夏裕宁,李雪梅,袁楠楠,张鑫磊,谭永星..高浓度氢气吸入对大鼠局灶性脑缺血再灌注损伤脑皮质神经细胞凋亡及凋亡相关蛋白表达的影响[J].广东医学,2016,37(24):3645-3649,5.基金项目
国家自然科学基金资助项目（编号81260206），广西自然科学基金资助项目（编号2013GXNSFAA019155，2015GXNSFAA139130），广西壮族自治区卫生厅自筹经费科研课题（编号Z2009038，Z2012402， Z2013497），广西医学科学实验中心开放基金专项项目 （）
