临床与病理杂志2016，Vol.36Issue(11)：1754-1757,4.DOI:10.3978/j.issn.2095-6959.2016.11.013
非小细胞肺癌胸水细胞块c-M ET基因扩增检测分析
Analysis of cell blocksc-MET amplification in pleural effusion of non-small cell lung cancer
摘要
Abstract
Objective: To investigate the clinical value of the cell blocks for gene ampliifcation detection of c-mesenchymal-epithelial transition (c-MET).Methods: Two hundred and iftfeen cases of cell block from pleural effusion of non-small cell lung cancer (NSCLC) were collected. Four hundred and four cases of tissue block were detected by RT-PCR method. hTe consistency ofc-MET ampliifcation was examined in 74 cases of patients with tissue block and cell block.Results:c-MET ampliifcation was found in 31 of 215 cell blocks (positive detection rate of 14.42%). c-MET ampliifcation was detected in 35 of 404 tissue blocks (positive detection rate of 8.66%). hTere were 68 cases in the 74 (91.89%) cases had the same consistency as tissue block.c-MET ampliifcation was detected in 9 of 74 (12.16%) cell blocks, and 13 of 74 (17.57%) tissue blocks.Conclusion: hTe rate ofc-MET ampliifcation in cell blocks of non-small cell lung cancer is higher than in matched tissue blocks. hTe patients with malignant pleural effusion are likely to tendc-MET ampliifcation.关键词
肺肿瘤/非小细胞肺癌/细胞块/c-METKey words
lung neoplasm/non-small cell lung cancer (NSCLC)/cell block/c-MET引用本文复制引用
朱礼阳,徐建平,于忠和,赵洁婷,叶伟,朱东波,孙晓,宋蓉蓉,许春伟..非小细胞肺癌胸水细胞块c-M ET基因扩增检测分析[J].临床与病理杂志,2016,36(11):1754-1757,4.基金项目
国家自然科学基金(81372489)；北京市科技计划课题(2131100006813032)。This work was supported by National Natural Science Foundation of China (81372489) and Beijing Municipal Science and Technology Plan Project (2131100006813032), P. R. China （81372489）
