国际病理科学与临床杂志2016，Vol.36Issue(12)：1900-1904,5.DOI:10.3978/j.issn.2095-6959.2016.12.002
绿茶提取物表没食子儿茶素没食子酸酯对酒精诱导SH-SY5Y细胞损伤的作用研究
Effect of green tea compound epigallocatechin-3-gallate on alcohol-induced SH-SY5Y cell injury
摘要
Abstract
Objective: To observe in vitro effects of green tea compound epigallocatechin-3-gallate (EGCG) on alcohol-induced SH-SY5Y cell injury and discuss the feasibility of alcohol-associated dementia (AAD) treatment with EGCG.Methods: SH-SY5Y neuron cells were treated with ethanol (100 mol/L) for 24 h in 37℃ for ethanol group. In EGCG + ethanol group, cells were treated with different doses of EGCG (1—100 µmol/L) and ethanol (100 mol/L) for 24 h in 37℃. For control group or EGCG group, cells were treated only with cell medium or same doses of EGCG. The cell viability was analyzed with MTT assay. The staining with Annexin-V APC/7- <br> AAD was used to observe cell apoptosis and the cell morphology was observed by optical microscope.Results:hTe cell survival was improved by treatment of EGCG for 24 h in a dose-dependent manner (25—100 µmol/L). Compared with control group (4.90%±0.80%) or EGCG group (7.82%±0.68%), cell apoptosis was apparently induced by treatment of ethanol (100 mol/L) for 24 h (21.82%±1.27%,P<0.001). However, co-treated with EGCG (100 µmol/L), these effects of ethanol on SH-SY5Y cells was prevented (10.81%±0.31%,P<0.001). Conclusion:EGCG inhibits alcohol-induced SH-SY5Y cell apoptosis and improve cell survival, which suggests that EGCG may have potential values for AAD treatment.关键词
表没食子儿茶素没食子酸酯/SH-SY5Y细胞/凋亡/酒精性痴呆Key words
epigallocatechin-3-gallate/SH-SY5Y cell/apoptosis/alcohol-associated dementia引用本文复制引用
杨海玉,刘勇,胡建新,丽丹,巢海潮,曾红..绿茶提取物表没食子儿茶素没食子酸酯对酒精诱导SH-SY5Y细胞损伤的作用研究[J].国际病理科学与临床杂志,2016,36(12):1900-1904,5.基金项目
江西省卫生厅中医药科研基金重点项目(2013Z005)。This work was supported by Jiangxi Province Health Department Traditional Chinese Medicine Key Project Foundation (2013Z005), P. R. China （2013Z005）
