华侨大学学报（自然科学版）2017，Vol.38Issue(1)：64-68,5.DOI:10.11830/ISSN.1000-5013.201701012
超滤法去除重组腺相关病毒中内毒素的应用
Removal of Endotoxin From Recombinant Adeno-Associated Virus by Ultrafiltration
摘要
Abstract
To explore the possibility of ultrafiltration technology used in recombinant adeno-associated virus purification,the recombinant adeno-associated virus sample termed rAAV9-Kal was first treated by different molecular weights cut off ultrafiltration devices,then the endotoxin and virus in the retentates and filtrates were assayed with quantitative tachypleus amebocyte lysate regents and real-time PCR respectively,and last the effects of ultrafiltration on endotoxin removal were evaluated.The results showed that the recovery rate and endotoxin removal rate of sample were 0.912 2 and 0.870 6 respectively,under the treatment of EDTA, DOC and 30 kD ultrafiltration devices.Therefore,the 30 kD should be the most suitable ultrafiltration device for sample ultrafiltration and endotoxin removal.关键词
重组腺相关病毒/超滤/截留分子量/内毒素/纯化Key words
recombinant adeno-associated virus/ultrafiltration/molecular weights cut off/endotoxin/purifi-cation分类
生物科学引用本文复制引用
唐明青,张文豪,侯莹,成志云,郭思佳,王立振,刘慧,许瑞安..超滤法去除重组腺相关病毒中内毒素的应用[J].华侨大学学报（自然科学版）,2017,38(1):64-68,5.基金项目
国家自然科学基金资助项目（2012AA020810）；福建省中青年教师教育科研项目（JA14020）；华侨大学科研基金资助项目 （）
