基础医学与临床2017，Vol.37Issue(1)：13-18,6.
持续低氧预处理促进兔尿源性干细胞增殖和减少凋亡
Sustained hypoxia promotes the proliferation and reduces apoptosis of rabbit urine derived stem cells
摘要
Abstract
Objective To investigate the effects of hypoxia on the cell proliferation , apoptosis and secretion of rab-bit urine derived stem cells ( rUSCs ) .Methods rUSCs were isolated and cultured with oxygen concentration of 20%and 3%, respectively .To observe the morphological changes of rUSCs in different culture conditions continu-ously;The growth curves under different culture conditions were drawn respectively;Antibody detection of factor se-cretion was performed;The cell cycle and the apoptosis were analyzed by flow cytometry .Results Single , small , compact “rice-grain” like cells were observed on day 6, after cultured in different oxygen concentrations ,the cells in normoxia group appeared like a “spindle”, the cells in hypoxia group changed to a “ pebble” morphology;The growth curves of two groups showed a “S” shape , but the cells in hypoxia group had a faster growth speed as com-pared with normoxia group ( P <0.01 resp.); Compared with normoxia group , the paracrine effect in hypoxia group had an increased expression level , and an increased variety;Less cells were arrested at G 0/G1 phase under hypoxia as compared with that in normoxia group (71.82%±8.86%and 91.73%±1.35%, resp.) (P<0.05), while the cell numbers at S phase increased in hypoxia group (18.77%±3.87% and 3.48%±0.73%, resp.) ( P<0.01);The apoptosis rate of normoxia group (3.24%±0.38%) was significantly higher than that of hypoxia group by flow cytometry (0.43%±0.05%) ( P<0.001 ); and the apoptosis rate of normoxia group (4.27%± 0.48%) was significantly higher than that of hypoxia group by Hoechst (1.37%±0.33%) ( P<0.01 ) .Conclusions Cell culture in 3%O2 could enhance cell proliferation and reduce apoptosis of rUSCs .关键词
兔尿源性干细胞/低氧/增殖/凋亡Key words
rabbit urine derived stem cells/hypoxia/cell proliferation/cell apoptosis分类
医药卫生引用本文复制引用
马文军,储加强,陶立,龚梦嘉,毕杨,魏光辉,张元原..持续低氧预处理促进兔尿源性干细胞增殖和减少凋亡[J].基础医学与临床,2017,37(1):13-18,6.基金项目
国家自然科学基金（81371704，81570650）；国家临床重点专科建设项目 （）
