基础医学与临床2017，Vol.37Issue(1)：56-61,6.
EGS-C67抑制HCV基因表达及其抗病毒活性的测定
Inhibiting effect of EGS-C67 on HCV gene expression and determination of its antiviral activity
摘要
Abstract
Objective To explore advantage of RNA guiding activity in vitro cleavage assay and intracellular antivi-ral effects for hepatitis C, basing on one external guide sequence (EGS)sequence of the conserved region of 5′UTR of HCV genome .Methods We analyzed the sequence and structure of the conserved region of 5′UTR of HCV ge-nome with computer software and designed one EGS which was direct at the potential targeting site ( C67-G68 ) .We tested the gene expression of HCV in Northern blot and Western blot and its antiviral activity by fluorescence quan -titative PCR in the Huh7.5.1 cell which was transfected by EGS-C67 .Results EGS-C67 had obvious advantage of RNA guiding activity in vitro cleavage assay .EGS-C67 obviously inhibited the gene expression of HCV and inhibit the proliferation of HCV and down-regulate the titer of HCV about 150 times( P<0.01 ) .Conclusions EGS-C67 , the new antiviral RNA has notably antiviral activity in cultured cells , thus could provide a new promising approach for development of anti-HCV strategy .关键词
丙型肝炎病毒/外部引导序列/抗病毒Key words
HCV/external guide sequence/antiviral分类
医药卫生引用本文复制引用
张成成,李小英,伍婧茹,张文军..EGS-C67抑制HCV基因表达及其抗病毒活性的测定[J].基础医学与临床,2017,37(1):56-61,6.基金项目
中国博士后科研基金 （）
