基础医学与临床2017，Vol.37Issue(1)：94-97,4.
高糖及高胰岛素对人血管平滑肌细胞增殖、迁移及miR-145水平的影响
Effects of both high glucose and high insulin on proliferations and migrations of human vascular smooth muscle cells as well as miR-145 level
金成吉 1唐田 2于晓静 1曲建梅 1段薇 1张弢1
作者信息
- 1. 大连大学附属中山医院内分泌科，辽宁大连116001
- 2. 聊城市人民医院内分泌科，山东聊城252000
- 折叠
摘要
Abstract
Objective To identify the effect of both high glucose and high insulin on miR-145 level.Methods The human vascular smooth muscle cells ( VSMCs) were cultured and the proliferation of VSMCs was induced by high glucose and high insulin medium.The samples were divided into 4 groups: normal group, high glu-cose group (25 mmol/L), high insulin group (300 mU/L), high glucose and high insulin group.The ex-pression of miR-145 in VSMCs was assayed by real-time PCR.Proliferation of VSMCs was determined by MTT method After 72 h cultivation.The migration of VSMCs was analyzed by cell scratch test .VSMCs in each group was transfected by miR-145 virus ( lentiviral vector ) .Proliferation and migration were assayed after 48 h transfection.Results The expression of miR-145 in VSMCs of other three groups was decreased ( P<0.05 ) , especially the expression in the high glucose and insulin group was the lowest ( P<0.01 ) .Prolifera-tion and migration of VSMCs was promoted by high glucose and/or high insulin medium.Under fluorescent, transfection rate of VSMCs was about 80%after 48 h transfection.Proliferation and migration of VSMCs in each group after transfection were significantly lower than before ( P<0.05 ) .Conclusions High glucose and high in-sulin could decrease the expression of miR-145 in VSMCs, The overexpression of miR-145 may inhibit the prolif-eration and migration of VSMCs .关键词
miR-145/血管平滑肌细胞/糖尿病Key words
miR-145/vascular smooth muscle cells/diabetes mellitus分类
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金成吉,唐田,于晓静,曲建梅,段薇,张弢..高糖及高胰岛素对人血管平滑肌细胞增殖、迁移及miR-145水平的影响[J].基础医学与临床,2017,37(1):94-97,4.
