军事医学2016，Vol.40Issue(12)：946-951,957,7.DOI:10.7644/j.issn.1674-9960.2016.12.003
一种简易的用于分析肿瘤细胞趋电性的微流控芯片技术
A simple microfluidic chip technology for assaying electrotaxis of cancer cells
摘要
Abstract
Objective To develop a simple microfluidic chip technology for analyzing the electrotaxis of cancer cells . Methods The basic structure of the proposed microfluidic electrotaxis chip included a straight microchannel and liquid storage pools located on both sides of the microchannel .Two platinum electrodes were inserted into the liquid pools to create a controllable direct current ( DC ) field in the microchannel .The distribution and strength of the DC field in the microchannel was analyzed by the finite element analysis software COMSOL multiphysics and experiment tests .Finally, the electrotactic behavior of the rhabdomyosarcoma RD cells in the DC field of different strength was characterized using the accumulated distance, average velocity, x forward migration index ( xFMI) and y forward migration index ( yFMI) as quantitative parameters.Results The results of element analysis and experiments showed that the structure of the designed microfluidic electrotaxis chip was able to guarantee a uniform and strength-controllable DC field in the microchannel .The experiment of cell electrotaxis showed that the RD cells migrated toward the anode of the DC field .Meanwhile , the values of xFMI and accumulated distance for RD cells increased with the enlargement of the DC field , with the strength ranging from 188 to 1320 V/m.Conclusion The microfluidic chip technology developed in this paper for assaying the electrotaxis of cancer cells is simple and easily implementable , and it can be used for studies of the electrotactic behavior and underlying mechanisms of various cancer cells and normal cells in the future .关键词
微流控芯片/电趋化/肿瘤迁移/横纹肌肉瘤RD细胞Key words
microfluidic chip/electrotaxis/tumor migration/rhabdomyosarcoma RD cell分类
医药卫生引用本文复制引用
米永华,肖文海,廖娟,陈静,李远..一种简易的用于分析肿瘤细胞趋电性的微流控芯片技术[J].军事医学,2016,40(12):946-951,957,7.基金项目
重庆市自然科学基金面上资助项目（cstc2012jjA10046）；重庆市卫生局中医药科技资助项目（ ZY20132132）；重庆市永川区创新能力建设平台资助项目（Ycstc2014bf5001）；重庆市大足区科技计划资助项目（DZKJ，2014ACC1084）；重庆医科大学附属永川医院院级重点研究资助项目 （）
