军事医学2016，Vol.40Issue(12)：952-957,6.DOI:10.7644/j.issn.1674-9960.2016.12.004
氨茶碱分子印迹电化学传感器的研制及应用
Preparation and application of aminophyllin electrochemical sensor based on molecularly imprinting technique
摘要
Abstract
Objective To establish a method for detection of aminophylline in blood samples of preterm infants . Methods A molecularly imprinted electrochemical sensing film on the glassy carbon electrode surface was prepared by electropolymerization using aminophylline as the template molecule and pyrrole as the functional monomer in 0.2 mol/L HAc-NaAc buffer solution ( pH 4.0).The surface morphology and properties of molecularly imprinted sensing films were characterized by three dimensional laser scanning microscopy , differential pulse voltammetry ( DPV) and electrochemical impedance spectroscopy ( EIS) while the effects of scanning cycle number and incubation time were investigated by square wave voltammetry(SWV) method in 5 mmol/L K3[Fe(CN)6] -0.1 mol/L KCl solution.Results Under optimized experimental conditions ,the SWV peak current difference was linear to the negative logarithm of aminophylline concentration in the range from 1.0 ×10 -7 to 1.0 ×10 -3mol/L with a detection limit (S/N=3) of 0.5 ×10 -8mol/L.The recovery rate was 92.2% -101.4%.Also, the molecularly imprinted electrochemical sensor for aminophylline had good selectivity , stability and reproducibility .Conclusion The molecularly imprinted electrochemical sensor for aminophylline can be used for rapid and accurate detection of clinical blood concentrations of aminophylline molecules in preterm infants in the future .关键词
分子印迹/氨茶碱/聚吡咯/电化学传感器Key words
molecular imprinting/aminophylline/pyrrole/electrochemical sensor分类
医药卫生引用本文复制引用
陈静,肖文海,廖娟,何庆梅,李远,龚放..氨茶碱分子印迹电化学传感器的研制及应用[J].军事医学,2016,40(12):952-957,6.基金项目
重庆市自然科学基金面上资助项目（cstc2012jjA10046）；重庆市卫生局中医药科技资助项目（ ZY20132132）；重庆市永川区创新能力建设平台资助项目（Ycstc2014bf5001）；重庆市大足区科技计划资助项目（DZKJ，2014ACC1084）；重庆医科大学附属永川医院院级重点研究资助项目 （）
