军事医学2016，Vol.40Issue(12)：978-983,6.DOI:10.7644/j.issn.1674-9960.2016.12.009
糖网明目颗粒对大鼠长期毒性研究
Repeated dose toxicity of Tangwang Mingmu granules in Sprague-Dawley rats
摘要
Abstract
Objective To observe toxic symptoms and signs , toxic damage extents and reversibility in rats after oral administration of Tangwang Mingmu granules .Methods Four dose groups with 40 rats in each group were designed in this study, including control group fed with distilled water and three groups at different dosages of the test drug .Tangwang Mingmu granules were orally administered to SD rats at the dosage of 8.4, 4.2 and 2.1 g/kg for 3 weeks and 14.0, 8.4 and 4.2 g/kg for 23 weeks, for 26 consecutive weeks .The general state of the rats was observed every day , while body mass and food consumption were calculated once a week .Halfway through and at the end of the administration (13 and 26 weeks) and after four weeks of recovery, parameters of body mass, hematology, hematological biochemistry, organ/body mass ratio and histopathology were measured .Results Compared with the control group at the same time-point, body mass of male rats in the other three groups was slightly reduced .Food consumption in high and medium dose groups was reduced (P<0.05), MCHC, ALT, TBIL and Na +in high dose group were decreased (P<0.05), TP, ALB and D-BIL were increased (P<0.05), the mean body mass and relative organ weight of thymus in medium dose male rats were decreased (P<0.05), relative organ weight of the liver and kidney in high dose male rats was increased (P<0.05), and focal chronic inflammation to different extent was observed in the liver , kidney and prostate gland .No dose-effect relationship was found in these perturbations that were all within the normal range of animals .No significant drug-related pathological changes were found.Conclusion The NOAEL of Tangwang Mingmu granules is considered to be 14.0 g/kg body mass/day (equal to 50 times the proposed clinical adult dosage ) for the 26-week repeated dose oral toxicity study in male andfemale rats.关键词
糖网明目颗粒/毒性/NOAEL/大鼠Key words
Tangwang Mingmu granules/repeated dose toxicity/no-observed-adverse-effect level (NOAEL)/Sprague-Dawley rats分类
医药卫生引用本文复制引用
彭辉,连增林,赵君,王以美,彭双清,赵增明,贾栗,束玉磊,何俊,张廷芬,李利忠,何秀姝,刘晔..糖网明目颗粒对大鼠长期毒性研究[J].军事医学,2016,40(12):978-983,6.基金项目
国家自然科学基金重点资助项目 （）
