军事医学2016，Vol.40Issue(12)：984-987,993,5.DOI:10.7644/j.issn.1674-9960.2016.12.010
骨形态发生蛋白9促进小鼠颅骨间充质祖细胞成骨分化的实验研究
BMP9 effectively induces osteogenic differentiation of immortalized calvarial mesen-chymal progenitor cells
摘要
Abstract
Objective To investigate the effect of adenovirus-bone morphogenic protein 9 ( Ad-BMP9 ) on osteogenic differentiation of immortalized calvarial mesenchymal progenitor cells ( iCALs ) .Methods iCALs were infected with adenoviral vectors encoding BMP-9 or green fluorescent protein ( GFP) and the early osteogenic differentiation was assessed by detecting alkaline phosphatase (ALP) activity after being cultured for 3, 5 and 7 days.14 days after infection, alizarin red S staining was performed to study the formation of osteogenic calcium nodules .The expression of osteogenic marker genes Runx2 and OCN was assessed by quantitative real-time ( RT )-PCR and Western blotting .Results Significant increases in ALP activity and in the expressions of Runx 2 and OCN were detected in BMP-9 treated iCALs compared with GFP-treated cells(P<0.05).Meanwhile, alizarin red S staining showed that more mineralized nodules were found in the BMP-9 induced group .Conclusion BMP-9 can promote the osteogenic differentiation of iCALs .关键词
骨形态发生蛋白质类/小鼠/颅骨间充质祖细胞/成骨分化/颅骨修复/碱性磷酸酶Key words
bone morphogenetic proteins/mouse/calvarial mesenchymal progenitor cells/osteogenic differentiation/repair of cranial defect/alkaline phosphatase分类
医药卫生引用本文复制引用
宿旭东,邓锋,汤寅虹,周鹏飞,梁盼盼,蓝丹,黄恩毅..骨形态发生蛋白9促进小鼠颅骨间充质祖细胞成骨分化的实验研究[J].军事医学,2016,40(12):984-987,993,5.基金项目
国家自然科学基金资助项目（81301551）；重庆市基础科学与前沿技术研究专项资助项目（cstc2013jcyjA10022）；重庆高校创新团队建设计划资助项目 （）
