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木霉菌M2的鉴定及其对小白菜促生效果研究

邢芳芳 高明夫 胡兆平 范玲超

农业环境与发展2017,Vol.34Issue(1):80-85,6.
农业环境与发展2017,Vol.34Issue(1):80-85,6.DOI:10.13254/j.jare.2016.0197

木霉菌M2的鉴定及其对小白菜促生效果研究

Identification of Trichoderma Strain M2 and Related Growth Promoting Effects on Brassica chinensis L

邢芳芳 1高明夫 1胡兆平 1范玲超1

作者信息

  • 1. 金正大生态工程集团股份有限公司/养分资源高效开发与综合利用国家重点实验室,山东 临沂 276700
  • 折叠

摘要

Abstract

The research took Trichoderma strain as tested strains which isolated from the rhizosphere of healthy and high yield pepper, M2 was classified and identified by combining morphological classification with molecular identification means(rDNA-ITS sequence analysis), whose effect on growth of Brassica chinensis L. was explored, in order to provide superior strains for bio-fertilizer development. After solid fermentation of Trichoderma M2, under the condition of the greenhouse, took some research on the growth promoting effect on Brassica chi-nensis L. of Trichoderma M2. The results showed that the strain M2 was identified as Trichoderma harzianum strain. M2 had obvious effects on promoting growth, the SPAD and edible leaf number of Brassica chinensis L. Adding of 5.0í109 cfu M2 had the best effect on increasing the biological yield. Compared with CK, fresh weight and dry weight was increased by 30.26%and 20.08%respectively. Followed by inocula-tion of 5.0í108 cfu M2, fresh weight and dry weight was increased by 18.33% and 12.46% respectively. Therefore, M2 showed evident promoting effect on Brassica chinensis L., and had potential application value.

关键词

哈茨木霉菌/分类鉴定/小白菜/促生

Key words

Trichoderma harzianum/classification and identification/Brassica chinensis L./growth promoting effect

分类

农业科技

引用本文复制引用

邢芳芳,高明夫,胡兆平,范玲超..木霉菌M2的鉴定及其对小白菜促生效果研究[J].农业环境与发展,2017,34(1):80-85,6.

基金项目

山东省科技重大专项(新兴产业) ()

农业环境与发展

OACSTPCD

1005-4944

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