药学研究2016，Vol.35Issue(12)：703-708,728,7.DOI:10.13506/j.cnki.jpr.2016.12.007
2，5-二酮-3，6-二（N-富马酰基-4-氨基丁基）哌嗪（FDKP）载胰岛素微球的制备及表征
Preparation and characterization of insuIin-Ioaded microspheres with FDKP
摘要
Abstract
Objective To prepare and characterize insulin-loaded FDKP microspheres.Methods Insulin-loaded mi-crospheres were prepared using FDKP as the carrier material.Box-Behnken experimental design was applied to optimize the formulation and preparation conditions to yield satisfactory drug-loading and particle size.The morphology of microspheres was recorded on a Scanning Electronics Microphotograph(SEM)and the interaction between FDKP and insulin was investi-gated using the Differential Scanning Calorimeter(DSC).ResuIts The optimal formulation and preparation conditions were as follows:stirring time 2.4 h;the ratio of FDKP and insulin(W/ W),15.97%;pH of suspension,4.56.The drug-loading and particle size of final product were 13.23% and 4.515 μm,respectively.The results from DSC study identified the interac-tion between FDKP and insulin.SEM images showed that insulin-loaded FDKP microspheres were sphere with rose shape and porosity,surface of drug - loaded microspheres was smooth,and insulin was filled into pores of blank FDKP microspheres.ConcIusion The insulin-loaded FDKP microspheres manufactured using the optimal formulation and prepa-ration conditions possess great appearance and high drug loading.关键词
FDKP/胰岛素/载药微球/Box-Behnken 试验设计/吸附作用Key words
FDKP/Insulin/Drug-loaded microspheres/Box-Behnken experimental design/Absorption function分类
医药卫生引用本文复制引用
杨晨,王祺玥,涂家生,孙春萌..2，5-二酮-3，6-二（N-富马酰基-4-氨基丁基）哌嗪（FDKP）载胰岛素微球的制备及表征[J].药学研究,2016,35(12):703-708,728,7.基金项目
科技部“十二五”重大专项（No.2014ZX09507001006）；国家自然科学基金（No.81501579）；江苏省自然科学基金 （）
