水生生物学报2017，Vol.41Issue(1)：18-25,8.DOI:10.7541/2017.3
稀有鲫热激蛋白70的分子克隆及表达特征分析
MOLECULAR CLONING AND EXPRESSION CHARACTERIZATION OF HEAT SHOCK PROTEIN 70 FROM GOBIOCYPRISRARUS
摘要
Abstract
Heat shock protein (HSP) 70, as a molecular chaperone and extensively studied protein in environ-mental toxicology, plays a key role in membrane translocation, protein degradation, and regulatory process. However, as toxicology and risk assessment of freshwater, characterizations of HSP70 in rare minnow, Gobio-cypris rarus, remain unknown. In this paper, we described the full-length cDNA of HSP70, designated as GrHSP70, by cloning through rapid-amplification of cDNA ends (RACE) technique from the liver of Gobio-cypris rarus for the first time. The GrHSP70 shares high nucleotide sequence homolog, corresponding to amino acid sequence homology (>95%) with other fish species by NCBI blast analysis. Through quantitative real-time PCR (RT-PCR), we obtained the distribution of GrHSP70 in thirteen tissues of the untreated Gobio-cypris rarus juveniles and its expression profile in liver after pentachlorophenol (PCP) exposure. The re-sults indicated that mRNA transcription level of GrHSP70 is tissue-dependent, with the highest expression in the gonad tissue. Moreover, GrHSP70 is significantly up-regulated in liver by a concentration/time-depen-dent manner after PCP exposure. In summary, our results suggest that GrHSP70 may serve as a very sensi-tive biomarker for a toxicology and risk assessment of freshwater pollution in China.关键词
稀有逗鲫/热激蛋白70/cDNA末端快速扩增/五氯酚/生物标志物Key words
Gobiocypris rarus/HSP70/Rapid-amplification of cDNA ends/Pentachlorophenol/Bio-marker分类
生物科学引用本文复制引用
黄书婷,刘秋萍,邓川,和野,熊力,牛纯青,陈韵,刘堰..稀有鲫热激蛋白70的分子克隆及表达特征分析[J].水生生物学报,2017,41(1):18-25,8.基金项目
Supported by the National Program on Key Basic Research Project (973 Program （）
2012CB723205) （）
the Financial Support Received from the National Natural Science Foundation of China (21147002) （21147002）
