水生生物学报2017，Vol.41Issue(1)：26-32,7.DOI:10.7541/2017.4
兴国红鲤ER基因克隆表达及EE2暴露对肝中ER表达的影响
CLONE AND EXPRESSION OF ER GENES IN XINGGUO RED CARP AND THEIR EXPRESSION IN LIVER UPON EE2 EXPOSURE IN JUVENILES
摘要
Abstract
The full-length cDNAs of estrogen receptors, ERα, ERβ, ERβ1 and ERβ2, were cloned from Xingguo red carp (Cyprinus carpio var singuonensis, Cs). The amino acid sequence of Cs ERs shared the highest identity with their counterparts of three cyprinid species. The ERs highly expressed in the liver, ovary and intestines of female Cs. In male Cs, ERαand ERβmRNAs were mainly expressed in liver, and ERβ1 and ERβ2 mainly observed in intestine and testis, respectively. The mRNA level of ERαmRNAs was significantly up-regulated in in liver of Juveniles (150 dph) ex-posed to different concentrations (0.01, 0.1 and 1 nmol/L) of 17α-ethinylestradiol (EE2) for 1 and 2 weeks. The hepatic ERβmRNA expression was dramatically increased by EE2 exposure, and ERβ1 mRNA expression was suppressed by EE2 for 1 and 2 weeks. The expression of ERβ2 mRNA was suppressed in the 1-week group with the highest suppres-sion at 0.01 nmol/L of EE2. This study showed that EE2 differently regulated the expression of four ERs genes in liver. Compared to ERβ, ERβ1 and ERβ2, ERαis a more sensitive biomarker for short-term (1—2 week) EE2 exposure.关键词
雌激素受体/兴国红鲤/EE2/实时荧光定量PCRKey words
Estrogen receptor/Cyprinus carpio var singuonensis/17α-Ethinylestradiol/Quantitative real-time PCR分类
生物科学引用本文复制引用
宋明月,单喜双,陈细华,岳华梅,叶欢,杨晓鸽,李创举..兴国红鲤ER基因克隆表达及EE2暴露对肝中ER表达的影响[J].水生生物学报,2017,41(1):26-32,7.基金项目
国家高技术研究发展计划项目(2011AA100401)资助[Supported by the National High Technology Research and Development Program of China (2011AA100401) （2011AA100401）
