水生生物学报2017，Vol.41Issue(1)：43-49,7.DOI:10.7541/2017.6
血红素加氧酶1在斑马鱼低氧应激中的保护作用研究
STUDIES ON THE PROTECTIVE ROLE OF ZEBRAFISH HO1 IN RESPONSE TO HYPOXIA
摘要
Abstract
Heme oxygenase 1 (Ho1) is the rate-limiting enzyme in the degradation of heme into biliverdin, carbon monoxide and free divalent iron. Abnormal expression of ho1 gene is associated with the development and progression of various human diseases, while functions of zebrafish Ho1 under hypoxia stress remain largely unknown. This study explored the protection role of zebrafish Ho1 during hypoxia exposure. The results showed that hypoxia significantly induced zebrafish ho1 mRNA in ZF4 cells, embryos, brain, gill, and liver but significantly decreased it in heart and kid-ney after 24h hypoxic exposure. Ho1 inhibition by ZnPPIX decreased ZF4 cell viability under hypoxia stress, enhanced Ho1 activity protected ZF4 cell from hypoxia stress-induced cell death. These results showed that Ho1 may protect zebrafish from hypoxia-induced cell apoptosis.关键词
斑马鱼/血红素加氧酶1/低氧/细胞活力/细胞凋亡Key words
Zebrafish/Heme oxygenase 1/Hypoxia/Cell viability/Cell apoptosis分类
生物科学引用本文复制引用
李艳丽,徐功玉,肖金文,李志,赵浩斌,周青春,桂建芳,钟雪萍..血红素加氧酶1在斑马鱼低氧应激中的保护作用研究[J].水生生物学报,2017,41(1):43-49,7.基金项目
国家自然科学基金项目(30972254) （30972254）
淡水生态与生物技术国家重点实验室开放课题(2013FB03)资助[Supported by the National Natural Science Foundation of China (30972254) （2013FB03）
the State Key Laboratory of Freshwater Ecology and Biotechnology (2013FB03) （2013FB03）
