水生生物学报2017，Vol.41Issue(1)：50-55,6.DOI:10.7541/2017.7
三角帆蚌热休克蛋白70基因克隆及其表达分析
CLONING AND EXPRESSION ANALYSIS OF HEAT SHOCK PROTEIN 70 GENE FROM HYRIOPSISCUMINGII
摘要
Abstract
In the present study, the cDNA sequence of Hyriopsis cumingii HSP70 (HcHSP70) was cloned by 3′rapid amplification of cDNA ends methods (3′-RACE) based on a long chain sequence of HcHSP70 and it was determined by high flux sequencing for transcriptome of H. cumingii blood cells, and its expression in the different tissues was detec-ted with quantitative real time polymerase chain reaction (qRT-PCR). Results showed that the full-length of HcHSP70 cDNA was 2298 bp long including a 1974 bp open reading frame (ORF) encoding a polypeptide of 657 amino acids that estimated molecular mass of 71.6 Ku and an isoelectric point of 5.67. Sequence comparison indicated that HcHSP70 shares the highest identity (91%) with HSP70 of Tegillarca granosa and Crassostrea gigas. HcHSP70 mRNA expressed in all 5 detected tissues (hepatopancreas, gonad, gill, mantle, muscle) with the highest expression in hepatopancreas. Both mRNA and protein level of HcHSP70 in gill significantly up-regulated at 37℃and rapidly re-duced to normal level under 40℃.关键词
三角帆蚌/热休克蛋白70/序列分析/基因表达/水温刺激Key words
Hyriopsis cumingii/Heat shock protein 70/Cloning/Gene expression/Thermal stress分类
生物科学引用本文复制引用
吴圣楠,隗黎丽,李海军,付建平,刘毅..三角帆蚌热休克蛋白70基因克隆及其表达分析[J].水生生物学报,2017,41(1):50-55,6.基金项目
国家自然科学基金项目(31060359) （31060359）
江西省自然科学基金(20122BAB204017)资助[Supported by the National Natural Science Foundation of China (31060359) （20122BAB204017）
the Natural Science Foundation of Jiangxi Province (20122BAB204017) （20122BAB204017）
