水生生物学报2017，Vol.41Issue(1)：65-78,14.DOI:10.7541/2017.9
中国大陆尼罗罗非鱼引进群体间遗传关系分析
ANALYSIS OF GENETIC RELATIONSHIP AMONG THE INTRODUCED POPULATIONS OF NILE TILAPIA (OREOCHROMISNILOTICUS) IN CHINESE MAINLAND
摘要
Abstract
To evaluate molecular genetic relationships, genetic differentiation and gene flow among the introduced po-pulations of Nile tilapia (Oreochromis niloticus) in mainland China, 12 pairs of polymorphic microsatellite primers were used to analyze genetic relationships among 10 populations of Oreochromis niloticus (one indigenous population in Egypt as the control group, nine introduced populations in mainland China as experimental group). The measures of genetic distance [DA, DC, DSW, (δμ)2] showed that the genetic distance between EGY and USA was smallest [DA=0.2174, DC=0.4140, DSW=0.8769, (δμ)2=22.6904]. The measures of DA and DC showed that the maximum genetic distance was between GD and XJF (DA=0.5851, DC=0.6789). The measures of DSW and (δμ)2 showed that the maxi-mum genetic distance was between USA and XJF [DSW=4.0907, (δμ)2=138.18]. The measures of FST and RST showed that genetic di-fferentiation between EGY and GD was the minimum (FST=0.0326, RST=0.0337), and genetic differentia-tion between XJF and LY were the maximum (FST=0.2098, RST=0.2655). There were significant genetic differences between all pairs of po-pulations (P<0.05). Clustering relationship between populations showed that WY, GD, EGY and USA population were clustered together;BL, LY and EW population were clustered together;JNM and GLD popula-tion were clustered together. XJF population was clustered into an independent branch. Bayesian clustering analysis showed that 10 populations were clustered into two categories with XJF, BL, LY and EW in the first category popula-tion and others in the second category. The results of phylogenetic tree and Bayesian clustering analysis were suppor-ted by analysis of molecular variance and principal components analysis. The average values of historical gene flow among populations were evaluated on the basis of FST and RST, which were 2.4427 and 2.1983, respectively. Results of recent gene flow among populations showed that the first generation migrants in each population was 0—7, accounting for 0 to 23.3%of total samples. Overall, significant genetic difference was detected among introduced populations of Nile tilapia in China. And there is some historical gene flow and recent gene flow among populations. This study provided a scientific basis for protection and utilization of germplasm resources of introduced populations of Nile tilapia in China.关键词
尼罗罗非鱼/引进群体/遗传关系/遗传分化Key words
Oreochromis niloticus/Introduced population/Genetic relationship/Genetic differentiation分类
生物科学引用本文复制引用
唐首杰,杨洁,王成辉,李思发,赵金良..中国大陆尼罗罗非鱼引进群体间遗传关系分析[J].水生生物学报,2017,41(1):65-78,14.基金项目
国家现代农业产业技术体系建设专项(CARS-49) （CARS-49）
水产动物遗传育种中心上海市协同创新中心(ZF1206) （ZF1206）
上海高校青年教师培养资助计划(ZZhy12004)资助[Supported by China Agriculture Research System (CARS-49) （ZZhy12004）
Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding (ZF1206) （ZF1206）
the Funding Scheme for Training Young Teachers in Colleges and Uni-versities in Shanghai (ZZhy12004) （ZZhy12004）
