水生生物学报2017，Vol.41Issue(1)：79-85,7.DOI:10.7541/2017.10
富集优势基因型的后备亲本筛选以及相关分子标记的遗传效应分析
CANDIDATE PARENTS SELECTION CONTAINING MULTIPLE GENOTYPES WITH HIGHER PERFORMANCE AND CORRELATED MOLECULAR MARKERS’ GENETIC EFFECTS ANALYSIS
摘要
Abstract
Marker assistant breeding, molecular breeding and genome wide breeding can shorten the generation inter-val of germplasm improvement and creation, speed up the selection response. Contrast with traditional best linear un-biased prediction (BLUP), they can achieve the higher genetic gain with the higher cost. Practically, together with BLUP, pre-selection of candidate individuals, low density array, they will become an optional choice under the conside-ration of selection cost and efficiency. So, in the present article, we focus on the effect of limited markers enrichment on the candidate population growth performance.e.g. body weight. For the body weight phenotype can not only be de-termined by one genotype, but also by multiple loci additive effect, dominant effect and epitasis. Thus, multiple loci ex-plored needed to do variance components analysis and identify the specific genetic effect of these loci. A total of 450 fish (parents and their offspring) were collected, which were from Nanquan farm in Freshwater Fisheries Research Cen-ter in Chinese Fishery Academy. A total of 5 microstallites primers and 4 primers used for functional gene regional amplification were selected to observe the corresponded genotypes of such individuals. Correlation analysis between ef-fective molecular markers and body weight of Huanghe carp new strain and genetic effect analysis of such markers were examined too in the present paper. The result showed that, microstallite Koi42 and 4 SNPs from the functional genes have significant effect on the carp’s body weight. 13 candidate parents which have over two genotypes with higher body weight were developed. These individuals have better growth performance compared to that without these perfect genotypes. Further analysis by multiple step-wise regressions was conducted in order to make sure which markers can be implicated in practical breeding together effectively. CO626, D-Loop 253 and Koi42 were left by both linear regres-sion analysis and AIC criteria, where Koi42 has the highest contribution to the body weight variation among 3 markers. It is that Koi42 genotypes is related with sex by Fisher exact test. Genetic analysis supply the information Koi42 plays its role by additive effect (P<0.1). Among many epitasis components, significant effect between D-Loop 253 additive effect and Koi42 additive effect was found. Through variance components partition analysis, additive variance account for 11.4%, and effect of epitasis between two loci had the higher percentage (77.5%). This told us 3 mar-kers listed above contribute to the body weight mainly by epitasis. All of these results illustrated that Koi42 with its interaction partner D-Loop 253 could be used to marker assistant breeding or multiple markers based co-selection.关键词
黄河鲤新品系/基因型/体重/关联分析/上位效应Key words
Huanghe carp new strain/Genotype/Body weight/Correlation analysis/Epitasis分类
生物科学引用本文复制引用
张成锋,苏胜彦,朱健,石连玉..富集优势基因型的后备亲本筛选以及相关分子标记的遗传效应分析[J].水生生物学报,2017,41(1):79-85,7.基金项目
农业部淡水水产生物技术与遗传育种重点实验室开放课题(FBB201401) （FBB201401）
“十二五”科技支撑计划“大宗淡水主养鱼类新品种选育”(2012BAD26B02) （2012BAD26B02）
中国水产科学研究院基本科研业务费(2016RC-LX03)资助[Supported by the Open Project of Key Laboratory of Freshwater Aquatic Biotechnology and Breeding in Ministry of Agriculture (FBB201401) （2016RC-LX03）
the Key Projects in the National Science&Technology Pillar Program during the Twelfth Five-Year Plan Period (2012BAD26B02) （2012BAD26B02）
Special Scientific Research Funds for Central Non-profit Institutes, Chinese Academy of Fishery Sciences (2016RC-LX03) （2016RC-LX03）
