水生生物学报2017，Vol.41Issue(1)：86-94,9.DOI:10.7541/2017.11
VE和L-肌肽对大菱鲆幼鱼生长、抗氧化、非特异性免疫及血清生化指标的影响
EFFECTS OF DIETARY VITAMIN E AND L-CARNOSINE ON GROWTH PERFORMANCE, ANTIOXIDANT STATUS, NON-SPECIFIC IMMUNITY AND SERUM BIOCHEMICAL INDICES IN JUVENILE TURBOT (SCOPHTHALMUS MAXIMUS)
摘要
Abstract
A 2×4 factorial experiment (2 vitamin E×4 L-carnosine) was conducted to investigate the effects of dietary vitamin E (0 and 75 mg/kg) and L-carnosine (0, 50 mg/kg, 100 mg/kg and 200 mg/kg) on growth performance, antio-xidant status, non-specific immunity and serum biochemical indices in juvenile turbot. Eight diets were formulated and randomly allocated into triplicate groups of fish [initial body weight of (14.00±0.02) g] in 24 cylindrical tanks (H:80 cm×Φ70 cm, water depth 50 cm) with 46 fish per tank for an 8-weeks trial. Results showed that fish weight gain rate (WGR) and specific growth rate (SGR) were significantly enhanced by dietary vitamin E (P<0.05) but not by L-carnosine less than 100 mg/kg. However, WGR, SGR and protein efficiency ratio (PER) were depressed whereas feed conversion ra-tio (FCR) was elevated significantly by 200 mg/kg L-carnosine (P<0.05). Statistical interactions between vitamin E and L-carnosine were observed in serum glutathione peroxidase (GSH-PX), catalase (CAT) activities, malondialdehyde (MDA) content, liver total antioxidant capacity (T-AOC), superoxide dismutase (SOD), and MDA (P<0.05). The highest GSH-PX activity was in the group of 50 mg/kg and 100 mg/kg L-carnosine, and the highest CAT activity was in the group of 100 mg/kg and 200 mg/kg L-carnosine and it showed no significant difference in group of 50 mg/kg (P>0.05). Liver T-AOC and SOD activities reached the top by 100 mg/kg L-carnosine and it showed no significant dif-ference by 50 mg/kg L-carnosine (P>0.05) or the 75 mg/kg vitamin E. Main effects analysis show that serum T-AOC, SOD and liver CAT activities were significantly elevated by dietary vitamin E (P<0.05), serum T-AOC was signifi-cantly elevated by dietary L-carnosine (P<0.05). Statistical interactions between vitamin E and L-carnosine were ob-served in serum complement C3 and LZM activities (P<0.05). Complement C3 reached the highest value in the group of 50 mg/kg L-carnosine and 75 mg/kg vitamin E (P<0.05). Serum total protein (TP) was not significantly affected by dietary vitamin E and L-carnosine (P>0.05). Serum total cholesterol (TCHO) and triacyglycerol (TG) contents were de-creased by vitamin E addition (P<0.05). Comparatively, TG was reduced by dietary L-carnosine with the lowest level by 50 mg/kg L-carnosine (P<0.05). These results suggest that 75 mg/kg vitamin E and 50 mg/kg L-carnosine is the best diet formulation (fish oil 70 g/kg, Soy lecithin 10 g/kg).关键词
大菱鲆/L-肌肽/VE/抗氧化性能/非特异性免疫性能Key words
Scophthalmus maximus/L-carnosine/Vitamin E/Antioxidant status/Non-specific immunity分类
农业科技引用本文复制引用
王晓艳,王际英,马晶晶,刘云,李宝山,孙永智,王世信,张利民..VE和L-肌肽对大菱鲆幼鱼生长、抗氧化、非特异性免疫及血清生化指标的影响[J].水生生物学报,2017,41(1):86-94,9.基金项目
国家海洋公益性行业科研专项(201205025) （201205025）
国家海洋生物产业水生动物营养与饲料研发创新示范平台(201503002) （201503002）
山东省科技发展计划(2014GHY115006)资助[Supproted by Public Science and Technology Research Funds Projects of Ocean (201205025) （2014GHY115006）
the Marine Biological Industry of China:Aquatic Animal Nutrition and Feed Research and Innovation Demonstration Platform (201503002) （201503002）
the Science and Technology Development Plan Project of Shandong Province (2014GHY115006) （2014GHY115006）
