水生生物学报2017，Vol.41Issue(1)：95-100,6.DOI:10.7541/2017.12
两种抗雌激素药物对雌性斑马鱼脂肪代谢的影响
EFFECTS OF TWO ANTI-ESTROGEN DRUGS EXPOSURE ON LIPID METABOLISM OF FEMALE ZEBRAFISH (DANIORERIO)
摘要
Abstract
In higher animals, estrogen can regulate lipid metabolism of females;however, whether estrogen regulates fish lipid metabolism is unclear. To explore effects of estrogen on lipid metabolism in zebrafish, letrozole (LET) and tamoxifen (TAM) were treated female zebrafish (0.14±0.01) g for 5 weeks to measure triglyceride (TG) concentrations in liver, visceral and muscle and the expressions of the hepatic genes related to estrogen and lipid metabolism. The re-sults showed that low dose LET significantly increased TG in liver and viscera but not in muscle. Low dose TAM did not impact No TG content in all tested tissues but high dose TAM significantly decreased liver TAG. LET and TAM significantly induced CYP19A mRNA, and low dose TAM decreased ERα. LET increased FAS but decreased MTP ex-pression. DGAT had an increase tendency, and CPT-1 and PPARαhad a decreased tendency. It suggests a dyslipi-demia symptom in the LET exposure groups. Low dose TAM reduced MTP and high dose TAM enhanced it (P<0.05). These results showed important role of estrogen in lipid metabolism in female zebrafish with a complex dosage-and tissue-dependent pattern.关键词
雌激素/脂肪代谢/来曲唑/他莫昔芬/斑马鱼Key words
Estrogen/Lipid metabolism/Letrozole/Tamoxifen/Zebrafish分类
农业科技引用本文复制引用
姚亚运,贾永义,孙胜香,吴思苹,张运妮,乔芳,张美玲,周忠良,杜震宇..两种抗雌激素药物对雌性斑马鱼脂肪代谢的影响[J].水生生物学报,2017,41(1):95-100,6.基金项目
农业部淡水渔业健康养殖重点实验室和浙江省鱼类健康与营养重点实验室开放课题(ZJK2014004) （ZJK2014004）
国家重大基础研究项目(2014CB138600) （2014CB138600）
国家自然科学基金(31472290) （31472290）
华东师范大学研究生科研创新实践项目(YJSKC201518)资助[Supported by the Key Laboratory of Freshwater Fisheries and Health Cultivation of the Ministry of Agriculture (ZJK2014004), the National Basic Research Program of China (2014CB138600), National Natural Science Foundation of China (31472290) and ECNU Research Innovation Foundation for Postgraduates(YJSKC201518) （YJSKC201518）
