水生生物学报2017，Vol.41Issue(1)：146-154,9.DOI:10.7541/2017.19
四种植物蛋白对中华绒螯蟹幼蟹生长性能、氨基酸沉积率和抗氧化酶活性的影响
EFFECTS OF FOUR COMMONLY USED PLANT PROTEIN SOURCES ON GROWTH PERFORMANCE, AMINO ACIDS RETENTION AND ANTIOXIDANT ENZYME ACTIVITIES IN JUVENILE CHINESE MITTEN CRAB, ERIOCHIERSINENSIS
摘要
Abstract
Chinese mitten crab (Eriocheir sinensis) is an importantly economic species for aquaculture in China due to its desirable taste and nutrient content. In the past decades, with the continuous expansion of crab farm and artificial feed development, the increasing demand for fish meal by aquaculture industry makes fish meal an increasingly expen-sive and limiting commodity. This study investigated the effects of dietary partial replacement of fishmeal (FM) by four commonly used plant protein sources on juvenile Chinese mitten crab, with a basic diet containing 50%fishmeal as the control, and other four diets with 50%fishmeal replaced by 30.50%fermented soybean meal (FSBM), 32.50%soybean meal (SBM), 28%cottonseed meal (CSM), and 39%rapeseed meal (RSM). Each diet was fed to four replicates of ju-venile E. sinensis (0.249±0.003 g) for 8 weeks. The results showed that no differences were found in weight gain, spe-cific growth rate, feed conversion ratio, protein efficiency, and protein retention when compared crab fed the three test diets (FSBM, SBM, CSM) with the FM diet. The weight gain of E. sinensis fed RSM was not different from crab fed FM, but was lower than crab fed SBM (P<0.05). Feed conversion ratio of E. sinensis fed RSM was higher than those fed FM, FSBM and SBM. Protein efficiency of E. sinensis fed RSM was lower than all other groups (P<0.05). Protein retention of E. sinensis fed RSM was lower than crab fed SBM or CSM (P<0.05).No differences were found in the con-tents of the whole crab body moisture, ash, crude lipid, and total essential amino acid retention rate of the four plant protein sources groups relative to the FM group. However, the whole crab crude protein content of crab fed RSM was lower than crab fed SBM or CSM (P<0.05). The total essential amino acid retention of crab fed RSM was lower than crab fed FSBM or CSM (P<0.05). Superoxide dismutase and glutathione peroxidase activities in both serum and hepa-topancreas of crab were not different when compared the groups of the four plant protein sources with the FM group. The serum malonaldehyde content of crab fed RSM was higher than crab fed all other diets (P<0.05). The results indi-cates that partial replacement of fish meal with FSBM, SBM or CSM will not lead to any negative effects on growth performance, amino acid retention and antioxidant enzyme activities in juvenile Chinese mitten crab. Thus, FSBM, SBM or CSM can be used as alternative plant protein sources to replace fishmeal in diet for Chinese mitten crab up to 30%. However, rapeseed meal is not a suitable protein source due to the lower protein digestibility, anti-nutritional factor contents. In case where rapeseed meal must be used in the diet of E. sinensis, it should be used in combination with other plant protein sources, and adequate process should be conducted to lower contents of the anti-nutritional factors.关键词
中华绒螯蟹/植物蛋白源/鱼粉/生长/氨基酸沉积/抗氧化酶Key words
Eriochier sinensis/Plant protein sources/Fishmeal/Growth/Amino acid retention/Antioxidant分类
农业科技引用本文复制引用
崔燕燕,张南南,马倩倩,陈晴,沈振华,杜震宇,陈立侨..四种植物蛋白对中华绒螯蟹幼蟹生长性能、氨基酸沉积率和抗氧化酶活性的影响[J].水生生物学报,2017,41(1):146-154,9.基金项目
公益性行业(农业)科研专项(201203065) （农业）
“十二五”国家科技支撑计划课题(2012BAD25B03) （2012BAD25B03）
国家自然科学基金项目(31572629,31172422) （31572629,31172422）
上海市中华绒螯蟹现代农业产业技术体系建设 （）
上海教育委员会研究基金(E03009)部分资助[Supported by Special Fund for Agro-scientific Research in the Public Interest (201203065) （E03009）
the National Science&Technology Pillar Program during the 12th Five-year Plan Period (2012BAD25B03) （2012BAD25B03）
the National Natural Science Foundation of China (31572629,31172422) （31572629,31172422）
Construction of modern agricultural industry technology system for Eriochier sinensis in Shanghai （）
Research Fund of Shanghai Education Committee (E03009) （E03009）
