水生生物学报2017，Vol.41Issue(1)：165-173,9.DOI:10.7541/2017.21
牛磺酸及相关氨基酸对大菱鲆幼鱼生长性能及TauT mRNA表达的影响
EFFECTS OF DIETARY TAURINE AND RELATED AMINO ACIDS ON GROWTH PERFORMANCE AND TAURINE TRANSPORTER (TAUT) MRNA EXPRESSION OF JUVENILE TURBOT (SCOPHTHALMUSMAXIMUSL.)
摘要
Abstract
A 10-week feeding experiment in indoor flowing-through water system was conducted to evaluate the ef-fects of taurine, methionine and cysteine in diets on growth performance and taurine transporter (TauT) mRNA expres-sion of juvenile turbot (Scophthalmus maximus L.) [initial weight:(7.90±0.07) g]. The basal diet was supplemented with 0, 1%, 2%taurine, 0.5%methionine and 0.5%cysteine (Control T-0, T-1, T-2, M-0.5 and C-0.5, respectively) to formulate five isonitrogenous and isolipidic diets. The results showed that the specific growth rate (SGR) and feed effi-ciency (FE) of T-1 and T-2 group were significantly higher than that of the control group (P<0.05), and the visceral in-dexes (VSI) of T-1 and T-2 group was significantly lower than that of the control group (P<0.05). Compared with T-0 group, the SGR and FE of M-0.5 group improved (P>0.05), and VSI decreased significantly (P<0.05), while condition factors (CF) increased significantly (P<0.05). Compared with T-0 group, the SGR of C-0.5 group decreased signifi-cantly (P<0.05), and no significant differences in FE, VSI and CF were observed (P>0.05) between the two groups. T-1 and T-2 group had significantly lower TauT mRNA relative expression (P<0.05) in liver, brain and eye tissues than those in T-0 group, and the TauT mRNA relative expression in liver, brain and eye tissues decreased significantly (P<0.05) with increasing dietary taurine level. On the contrary, the TauT mRNA relative expression in liver, brain and eye tissues of M-0.5 group was significantly higher (P<0.05) than those in T-0, T-1 and T-2 groups. C-0.5 group had a significantly higher TauT mRNA relative expression (P<0.05) in liver, brain and eye than those in T-0, T-1 and T-2 groups, but no significant differences (P>0.05) were found when compared with M-0.5 group. In conclusion, 0.48%, 1.06%taurine in diets could promote growth performance of juvenile turbot under these experimental conditions. The TauT mRNA expression in juvenile turbot may be affected by dietary sulfur-containing amino acids such as taurine, methionine and cysteine.关键词
牛磺酸/蛋氨酸/半胱氨酸/大菱鲆/牛磺酸转运载体(TauT)Key words
Taurine/Methionine/Cysteine/Turbot/Taurine transporter (TauT)分类
农业科技引用本文复制引用
柳茜,梁萌青,郑珂珂,王成强,徐后国..牛磺酸及相关氨基酸对大菱鲆幼鱼生长性能及TauT mRNA表达的影响[J].水生生物学报,2017,41(1):165-173,9.基金项目
国家自然科学基金(31340076) （31340076）
农业公益行业专项(201303053)资助[Supported by the National Natural Science Foundation of China (31340076) （201303053）
the Special Fund for Agro-scientific Research in the Public Interest (201303053) （201303053）
