天津中医药2017，Vol.34Issue(1)：43-47,5.DOI:10.11656/j.issn.1672-1519.2017.01.11
加味四妙勇安汤对CIA大鼠关节炎性病理损伤的作用研究
Study on pathological and molecular mechanism of the effect of the modified Simiao Yong’an decoction on the arthritis in CIA rats
摘要
Abstract
Objective] To explore the effect of modified Simiao Yong’an decoction on collagen induced arthritis about arthritis index (AI), the joint tissue inflammatory pathological manifestations, and effect on protein expression of IL-6,IL-17,TNF-αin rats. [Methods] The successful CIA models of SD rats were randomly divided into model group, positive group (leflunomide), and high, medium, low dose group of Chinese medicine, and normal control group, 6 rats in each group. The intervention and treatment period was 12 weeks. AI val-ues of each rat were evaluated every week. At 12 weeks, rats were executed and right ankle joint of each group of rats was taken. Joint pathlological changes of each right ankle were observed. At the same time levels of IL-6, IL-17 and TNF-αin serum were examined by ELISA, mRNA transcription levels of IL-6, IL-17and TNF-αin synovial were detected by RT-PCR. [Results] AI scores of the three Chi-nese medicine groups decreased significantly (P﹤0.05 or P﹤0.01) in 6~12 weeks compared with the model group, and in the 8~10 weeks AI scores were even improved significantly than positive group (P﹤0.05 or P﹤0.01). Histological examination showed that the ankle joint cartilage pathological had an improvement in the medium dose of modified Simiao Yong’an decoction group compared with the control model group. Compared with the model group, mRNA and protein expression of IL-6, IL-17and TNF-αwere down regulated in all treat-ment groups significantly (P﹤0.01). [Conclusion] The anti-inflammatory action mechanism on CIA rats of modified Simiao Yong’an de-coction might achieve by reducing expression of IL-6, IL-17 and TNF-α.关键词
加味四妙勇安汤/CIA模型/AI/病理改变/分子机制Key words
modified Simiao Yong’an decoction/CIA model/AI/pathological change/molecular mechanism分类
医药卫生引用本文复制引用
马卫国,孟凤仙,张继胜,陆妍,王亚南,沈志明,王洁,刘慧..加味四妙勇安汤对CIA大鼠关节炎性病理损伤的作用研究[J].天津中医药,2017,34(1):43-47,5.基金项目
北京中医药大学青年教师自主课题（2015-JYB-JSMS119）。 （）
