天津中医药2017，Vol.34Issue(1)：48-53,6.DOI:10.11656/j.issn.1672-1519.2017.01.12
心复康丸对压力超负荷大鼠心肌组织microRNA-1影响的实验研究
Experimental study of Xinfukang pills on microRNA-1 in cardiac tissue of pressure overloaded rats
摘要
Abstract
Objective] To explore improving effect of Xinfukang pills on pressure overload-induced myocardial hypertrophy and ventricular remodeling in rats, the relationship between its pharmacology effect and the express of miR-1 and in cardiac tissue was evaluated. [Methods] The experiment adopted the method of narrowing and contraction the aorta in adult and healthy male Wistar rats to establish the model of animals with myocardial hypertrophy and ventricular remodeling. Four weeks after coarctation of abdominal aorta, the Wistar rats were randomly divided into model group (M group), Enalapril group(E group), Xinfukang pills low dose group(L group) and Xinfukang pills high dose group (H group). Sham surgery group did not ligate the abdominal aorta, other treatments were same as others. 8 and 12 weeks after surgery, myocardial tissue collagen volume fraction was evaluated by Masson dyeing. The express of SERCA2 and Fibullin-2 in cardiac tissue were detected by immunohistochemistry. The express of miR-1 was also detected by Real time PCR. [Results] The 8 and 12 weeks after surgery, compared with group M, the myocardial fibrosis area of group E and H were reduced significantly (P﹤0.01), and the express of Fibullin-2 in the cardiac tissue of group H was decreased significantly (P﹤0.01). The express of SERCA2 in the cardiac tissue of group H was increased significantly (P﹤0.01). The express of miR-1 in the cardiac tissue of group H increased significantly (P﹤0.01). After 8 weeks, the express of Fibullin-2 in the cardiac tissue of group L was different with group M (P﹤0.05). [Conclusion] Xinfukang pills can increase the express of miR-1 and regulating the target protein Fibullin-2, therefore it may inhibit apoptosis of myocardial fibrosis, and plays an important role in repressing cardiac hypertrophy and ventricular remodeling process.关键词
心复康丸/microRNA-1/压力超负荷/心肌肥厚Key words
Xinfukang pill/microRNA-1/pressure overload/myocardial hypertrophy分类
医药卫生引用本文复制引用
梁勇,郭鹏,陈作,李艳灵,刘永宇,孙玲,曹书华,高克俭..心复康丸对压力超负荷大鼠心肌组织microRNA-1影响的实验研究[J].天津中医药,2017,34(1):48-53,6.基金项目
天津市北辰区科技发展计划项目（bcws2013-08）。 （）
