天津中医药2017，Vol.34Issue(1)：54-58,5.DOI:10.11656/j.issn.1672-1519.2017.01.13
桂皮醛对滑膜成纤维细胞增殖、凋亡及周期影响的实验研究
Experimental research on the influence of cinnamic aldehyde on the proliferation, apoptosis and cell cycle of fibroblast-like synovial cells
摘要
Abstract
Objective] To investigate the influence of different concentrations of cinnamic aldehyde (CA) on the proliferation, apoptosis and cell cycle of fibroblast-like synovial cells (FLS), and then explore the regulatory mechanism of CA to the synovial inflammation in os-teoarthritis. [Methods] Different concentrations of CA were intervened on LPS-induced FLS respectively, and the proliferation, apoptosis and cell cycle were measured through Methylthiazolyldiphenyl-tetrazolium bromide assay (MTT) and flow cytometry(FCM). [Results] Low concentration of CA could promote the growth of FLS mildly, while high concentration of CA could significantly reduce the survival rate (P﹤0.05) in concentration-dependent manner. LPS could reduce the apoptosis rate of FLS, shorten the G1 phase and extend the S phase, it meant that the inflammatory model was successfully established. Compared with the control group, both high and low concentration of CA groups could obviously increase the ratio of cells in the areas of Q2 and Q4 (P﹤0.05), and extend the G1 phase and S phase (P﹤0.05) to in-duct the apoptosis and inhibit the proliferation of FLS, especially the effect of high concentration of CA group was more significant. [Conclusion] Different concentrations of CA has certain inhibition for the growth of FLS, and the high concentration of CA group has stronger inhibitory effect, which may be the potential mechanism of CA’s anti-inflammatory effects.关键词
骨关节炎/桂皮醛/滑膜成纤维细胞/增殖/流式细胞术Key words
osteoarthritis/cinnamic aldehyde/fibroblast-like synovial cell/proliferation/flow cytometry分类
医药卫生引用本文复制引用
王欢,甘稳,王庆甫,杨黎黎,张栋,王伟利,丁昊彬,郭玉茹,杜汪洋,徐铭康..桂皮醛对滑膜成纤维细胞增殖、凋亡及周期影响的实验研究[J].天津中医药,2017,34(1):54-58,5.基金项目
国家自然科学基金面上项目（No.81373662）；北京中医药大学自主选题项目（No.2014-JYBZZ-XS-171）。 （）
