天津中医药2017，Vol.34Issue(1)：62-65,4.DOI:10.11656/j.issn.1672-1519.2017.01.15
藤黄酸纳米结构脂质载体的制备及抗肿瘤作用初步评价
The preparation and preliminary evaluation of anti-tumor effect of Gambogic acid nanostructure lipid carrier
摘要
Abstract
Objective] To prepare the nanostructure lipid carriers (NLC) incorporated gambogic acid (GA) , investigate its physical and chemical properties and evaluate the anti-tumor effect preliminarily. [Methods] Emulsification-evaporation was used to prepare the nanoparticles. The particle size, Zeta potential, encapsulation efficiency were selected as the evaluation indexs to appraise the physical and chemical properties and process validation was implemented by using differential scanning calorimetry (DSC). The CCK-8 assay was used to measure human breast cancer cells MDA-MB-231 cell growth repression rate. [Results] The GA-NLC was about 20 nm in diame-ter. Zeta potential was-(5.86±0.64) mV. The encapsulation efficiency was (99.46±0.23)%. DSC results showed that GA exists in gambog-ic acid nanostructure lipid carrier in the form of amorphous. [Conclusion] The GA-NLC prepared by emulsification-evaporation was characterized of smaller particle size, uniform distribution and high encapsulation efficiency. Compared with GA solution, GA-NLC has a significant antitumor activity.关键词
藤黄酸/纳米结构脂质载体/理化性质评价/抑瘤作用Key words
gambogic acid/nanostructure lipid carrier/physical and chemical properties/anti-tumor effect分类
医药卫生引用本文复制引用
黄瑞,郭盼,刘勃缨,丁苗苗,张兵,张丽娜,刘志东..藤黄酸纳米结构脂质载体的制备及抗肿瘤作用初步评价[J].天津中医药,2017,34(1):62-65,4.基金项目
天津市自然科学基金（一般项目）（16JCYB-JC28200）；国家自然基金（面上项目）（81673605）。 （）
