生态环境学报2016，Vol.25Issue(10)：1727-1732,6.DOI:10.16258/j.cnki.1674-5906.2016.10.019
具重金属抗性产酸菌的分离及生物学特性研究
Study on Isolating of the Strain Having the Ability of Heavy Metal Resistance and Producing Organic Acid
摘要
Abstract
The soil in the surrounding area of Pb/Zn smeltery was contaminated by heavy metals such as Pb, Zn, Cd and Cu et al. The remediation technology for single heavy metal contaminated soil is already mature, but it needs further development for the seriously heavy metals compound contaminated soil. In the paper, the soil was collected in the surrounding area of Pb/Zn smeltery, and the microbiology having not only the ability of heavy metal resistance but also the ability of producing acid would be selected and isolated in order to being used to remedy the contaminated soil. Through field sampling and surveying, molecular biological identification, microscopic examination, a strain was selected from the soil samples which was indentified asAspergillusniger and named as F2, the ability of heavy metal resistant and bioleaching remediation was studied in the paper. The results show: The largest concentration of resistance to Pb, Zn, Cd and Cu ofAspergillusniger F2is 8500, 6000, 2200 and 2500 mg·L-1, the largest concentration of resistance to the compound of Pb, Zn, Cd and Cu is 105, 2400, 2200 and 2400 mg·L-1. The growth ofAspergillus nigerF2 on LB medium containing high concentrations of heavy metals was faster in the later stage than in the early stage. The pH value of liquid medium was degraded from 7.00 to 2.40 in 7 days. 2.5 g sterilized soil was added into 49 mL liquid medium, 1 mL spore suspension liquid was inoculated, then put in 28 ℃ and 120 r·min-1orbital shaker incubator, after 15 days, the bioleaching efficiency of Pb, Zn, Cd and Cu was 46.79%, 52.01%, 29.40% and 75.70% respectively, the total bioleaching efficiency of Pb, Zn, Cd and Cu was 59.54%. ThusAspergillusnigerF2 has large ability of heavy metal resistance and good applied value of bioleaching remediation on heavy metal contaminated soil.关键词
黑曲霉/重金属抗性/土壤/浸出修复Key words
Aspergillus/niger/heavy metal resistance/soil bioleaching分类
资源环境引用本文复制引用
邓新辉,彭扶风,陈韵声,何权运,孔乐群,李贝贝,张霞..具重金属抗性产酸菌的分离及生物学特性研究[J].生态环境学报,2016,25(10):1727-1732,6.基金项目
国家自然科学基金项目（51474102）；湖南省自然科学基金项目（2015JJ3059）；湖南省大学生创新项目 （）
