现代医药卫生2017，Vol.33Issue(1)：37-39,3.DOI:10.3969/j.issn.1009-5519.2017.01.014
Tat蛋白对人脑内皮细胞闭锁蛋白表达的影响
Effect of Tat protein on occludin in human brain endothelial cells
摘要
Abstract
Objective To investigate the effect of recombinant Tat protein on occludin in brain endothelial cells. Methods Human brain microvascular endothelial cells(HBMECs) were isolated and treated according to the Weksler′s method. The levels of gene mRNA transcripts were analyzed by one-step quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Occludin expression was detected by Western blot according to Reyes′s method. Immunofluorescence detection was con-ducted. The images were observed and analyzed by fluorescence microscope. Results The recombinant Tat protein could reduce occludin transcription and protein expression. The occludin mRNA level began to appear obvious inhibition after 30 min of re combinant Tat protein treatment(P<0.05). The occludin mRNA levels at 1,2,6,12,24 h of recombinant Tat protein treatment were 0.66,0.55,0.58,0.54,0.53 respectively,showing statistical differences compared with the control group (P<0.05). The occludin level maintain unchanged within 6 h,which was 0.71 and 0.61 at 12,24 h(P<0.05). Meanwhile,the histological alterations of occludin were also observed by fluorescence microscope. In the untreated group,occludin staining was strong and uniformly. After Tat treating cells for 24 h,the occludin staining was weakened obviously. Conclusion Recombinant Tat protein can disrupt blood brain barrier(BBB) integrity and inhibits occluding expression.关键词
血脑屏障/基因产物,tat/蛋白质类/脑/内皮细胞Key words
Blood-brain barrier/Gene products,tat/Proteins/Brain/Endothelial cells引用本文复制引用
徐瑞芬,赵清侠,吴刚,郭姣,侯敏娜,冯旭阳..Tat蛋白对人脑内皮细胞闭锁蛋白表达的影响[J].现代医药卫生,2017,33(1):37-39,3.基金项目
陕西省社会发展科技攻关项目（2016SF-045）。 （）
