湖北科技学院学报（医学版）2016，Vol.30Issue(6)：461-463,3.DOI:10.16751/j.cnki.2095-4646.2016.06.0461
丹参酮ⅡA 对成骨细胞增殖的影响
Effect of TanshinoneⅡA on the Proliferation of Osteoblasts
叶茂 1郑勇 1刘艳西 1陈明 1胡锋1
作者信息
- 1. 湖北科技学院附属第一医院脊柱关节外科，湖北咸宁437100
- 折叠
摘要
Abstract
Objective To investigate the effect of Tanshinone ⅡA on the proliferation of osteoblasts . Methods The osteoblasts were cultured in vitro and divided into four groups:control group ,low dose of Tanshi-none ⅡA group(5 μM),middle dose of Tanshinone ⅡA group(10 μM) and high dose of Tanshinone ⅡA group(20μM).The proliferation activity of osteoblasts was detected with CCK-8 kit.Intracellular alkaline phos-phatase activity was determined by two ethanol amine method .Apoptosis rate of osteoblasts was detected by flow cytometry.Results The proliferation activity was significantly increased in middle and high doses of Tanshinone II A groups and there was statistical significance as compared to the control group (P<0.05 or P<0.01). Compared with the control group ,the activity of alkaline phosphatase activity in high dose group was increased (P<0.05),while the apoptosis rate was decreased( P<0.05).Conclusion Tanshinone II A can promote the proliferation of osteoblasts partly by inhibiting the apoptosis of osteoblasts ,which may provide experimental evi-dence for the treatment of osteoporosis .关键词
丹参酮ⅡA/成骨细胞/增殖/凋亡Key words
Tanshinone ⅡA/Osteoblast/Proliferation/Apoptosis分类
医药卫生引用本文复制引用
叶茂,郑勇,刘艳西,陈明,胡锋..丹参酮ⅡA 对成骨细胞增殖的影响[J].湖北科技学院学报（医学版）,2016,30(6):461-463,3.
