新型炭材料2016，Vol.31Issue(6)：639-645,7.
氧化石墨烯对L-色氨酸荧光猝灭及机理研究
The mechanism of the fluorescence quenching of L-tryptophan by graphene oxide
摘要
Abstract
Graphene oxide ( GO) was prepared by the modified Hummers method and used as a fluorescence quenching reagent of L-tryptophan ( L-Trp) . The structure of GO was characterized by FT-IR and Raman spectroscopy. The binding mechanism of L-Trp on GO and their reaction were investigated by UV spectroscopy and the fluorescence method. Results show that GO has a strong flu-orescence quenching ability for L-Trp. The quenching efficiency is 95. 6% for L-Trp with a concentration of 2×10-5 mol·L-1 when the GO concentration is 16×10-5 g·mL-1. The binding constants of L-Trp on GO were 2. 03×105, 1. 83×105 and 1. 63×105 mL·g-1 at 25, 30 and 35℃, respectively, as revealed by the Lineweawer-Burk equation, indicating a strong interaction between GO and L-Trp. GO quenches the intrinsic fluorescence of L-Trp by the formation of a complex, and is a static quenching mechanism. The quenching rate constant was 6. 65×1012 mL·(g·s)-1 at 25 ℃ as determined by the Stern-Volmer equation.关键词
氧化石墨烯/L-色氨酸/荧光猝灭Key words
Graphene oxide/L-tryptophan/Fluorescence quench分类
化学化工引用本文复制引用
弓巧娟,王永东,杨海英,原梅妮..氧化石墨烯对L-色氨酸荧光猝灭及机理研究[J].新型炭材料,2016,31(6):639-645,7.基金项目
山西省“131”领军人才工程项目 （）
山西省自然科学基金项目(2012011007-1) （2012011007-1）
运城市科技局项目(2060499).@@@@Financial support from the Science Foundation of “131” Leading talents project of Shanxi Province. （2060499）
Science Foundation of Shanxi (2012011007-1) （2012011007-1）
Science Foundation of Bureau of Science and Technology of Yuncheng (2060499) （2060499）
