中国病理生理杂志2017，Vol.33Issue(1)：7-12,6.DOI:10.3969/j.issn.1000-4718.2017.01.002
ENDOD1在前列腺癌组织及细胞中的表达及意义
Value of endonuclease domain containing 1 in progression of prostate cancer
摘要
Abstract
AIM: To analyze the difference of endonuclease domain containing 1 (ENDOD1) expression be-tween benign prostatic hyperplasia ( BPH ) tissues and prostate cancer ( PCa ) tissues and to investigate the effect of ENDOD1 on the biological function of human prostate cancer cells .METHODS: The BPH samples ( n=20 ) and PCa samples (n=21) were processed and analyzed according to the instruction of immunohistochemical (IHC) staining.The mRNA and protein levels of ENDOD 1 in the normal prostate epithelial cells and prostate cancer cells were evaluated by RT -qPCR and Western blot , respectively .The recombinant plasmids pCMV-N-Flag-ENDOD1 was constructed and was trans-fected into the human prostate cancer cells .The proliferation , apoptosis , migration and invasion abilities of the prostate cancer cells were evaluated by MTT assay , flow cytometry, Transwell migration and Matrigel invasion assays , respectively. RESULTS:The analysis of variance of the immunoreactivity score showed that PCa tissues with high Gleason score dis -played significantly lower ENDOD1expression than that with low Gleason score and BPH (P<0.05).The expression of ENDOD1 at mRNA and protein levels in PC3 cells and DU145 cells was significantly lower than that in the LNCap cells (P<0.05).The proliferation of DU145 transfected with ENDOD1 was inhibited.The flow cytometry indicated that ENDOD1 over-expression in the DU145 cells resulted in a notable increase in G0/G1 phase arrest (P<0.05), but the ap-optotic rates showed no statistical difference .The results of Transwell assay showed that migration and invasion abilities of the cells were also inhibited after transfection with over-expressing ENDOD1 plasmid (P<0.05).CONCLUSION: The expression of ENDOD1 significantly decreased in prostate cancer with high Gleaon score .Meanwhile, the ENDOD1 is spe-cifically down-regulated in androgen independent prostate cancer (AIPC) cell lines.Over-expression of ENDOD1 remark-ably inhibits the proliferation , migration and invasion abilities of AIPC .关键词
前列腺肿瘤/核酸内切酶结构域内含蛋白1/肿瘤侵袭Key words
Prostate cancer/Endonuclease domain containing 1/Neoplasm invasion分类
医药卫生引用本文复制引用
彭叔彬,曾花,邱剑光,胡成,黄文涛,李科,王德娟..ENDOD1在前列腺癌组织及细胞中的表达及意义[J].中国病理生理杂志,2017,33(1):7-12,6.基金项目
国家自然科学基金资助项目（No．81402111）；广东省自然科学基金资助项目（No．2015A030313031）；广东省医学科研基金资助项目 （）
