中国病理生理杂志2017，Vol.33Issue(1)：13-17,5.DOI:10.3969/j.issn.1000-4718.2017.01.003
地西他滨抑制 K562白血病细胞增殖和诱导分化的作用
Effect of decitabine on proliferation and differentiation of K562 cells
摘要
Abstract
AIM:To investigate the effect of decitabine (Dacogen, DAC) on the proliferation and differentia-tion of K562 cells.METHODS:The K562 cells were treated with different concentrations of DAC .The colony formation ability of the cells was detected by the colony formation assay with semi-solid culture .The cell viability was detected with MTT assay.The morphologic features were observed under inverted microscope with Wright ’s staining.The changes of the cell cycle distribution and the expression of CD 11b and CD42b were analyzed with flow cytometry .The protein expression of CDK2, cyclin E1, P27, GATA-1 and PU.1 in the K562 cells was determined by Western blot .RESULTS:DAC signifi-cantly decreased the colony number of the cells and cell viability in a dose-dependent manner .The morphological changes of the cells displayed partial differentiation .After treated the K562 cells with DAC for 72 h, the cell proportion in S phase was obviously decreased , while the cell proportion in G 2/M phase was obviously increased in a dose-dependent manner . After treated the K562 cells with DAC for 7 d, the percentage of CD11b and CD14 positive cells was further elevated , and the protein expression of P27, GATA-1 and PU.1 was increased.However, the protein expression of CDK2 and cyclin E1 was decreased .CONCLUSION:DAC inhibits the proliferation and induces differentiation of the K 562 cells via regulation of cell cycle .关键词
地西他滨/白血病/细胞增殖/分化/细胞周期Key words
Decitabine/Leukemia/Cell proliferation/Differentiation/Cell cycle分类
医药卫生引用本文复制引用
余潇苓,赵燕娜,郑智茵,高瑞兰,尹利明..地西他滨抑制 K562白血病细胞增殖和诱导分化的作用[J].中国病理生理杂志,2017,33(1):13-17,5.基金项目
国家自然科学基金资助项目（No．81373876）；浙江省自然科学基金资助项目 （）
