中国病理生理杂志2017，Vol.33Issue(1)：38-45,8.DOI:10.3969/j.issn.1000-4718.2017.01.007
BCL6B对人结直肠癌LoVo细胞增殖和迁移的影响及机制
Effects of BCL6 B on proliferation and migration of human colorectal car-cinoma LoVo cells and its potential mechanism
谷月 1李爱芳 1孙晖 1李雪茹 1查何 1赵佳丽 1谢佳卿 1周兰1
作者信息
- 1. 重庆医科大学检验医学院，临床检验诊断学教育部重点实验室，重庆400016
- 折叠
摘要
Abstract
AIM:To detect the endogenous expression of B-cell leukemia/lymphoma 6 member B (BCL6B) in FHC and LoVo cells, and to investigate the effects of BCL6B on proliferation and migration of LoVo cells for further explo-ring the underlying mechanism .METHODS:The endogenous expression of BCL 6B in the FHC and LoVo cells was detec-ted by RT-PCR and Western blot .The methods of MTT assay , colony formation assay , wound healing assay and Transwell chamber experiment were employed to examine the biological functions of BCL 6B in the LoVo cells.The mRNA and protein levels of BCL6B, cyclin D1 and matrix metalloproteinase-9 ( MMP-9) were determined by RT-PCR and Western blot , re-spectively.The level of phosphorylated protein kinase B (p-AKT) was detected by Western blot.RESULTS:BCL6B ex-pression was notably repressed in the LoVo cells as compared with the FHC cells , which were significantly increased by transfection with pcDNA3.1-BCL6B.The abilities of proliferation and migration of the LoVo cells at 72 h were inhibited by 28.33%(P<0.01) and 36.11%(P<0.05) in BCL6B group.The mRNA levels of cyclin D1 and MMP-9 in the cells of BCL6B group were decreased by 39.90%(P<0.01) and 77.36% (P <0.05), and the protein levels of cyclin D1, MMP-9 and p-AKT were reduced by 44.00%(P<0.05), 47.06%(P<0.01) and 32.88% (P<0.05), respectively. CONCLUSION:BCL6B inhibits proliferation and migration of the LoVo cells , and the PI3K/AKT signaling pathway is in-volved in this process .关键词
BCL6B/结直肠癌/细胞增殖/细胞迁移/PI3K/AKT信号通路Key words
BCL6B/Colorectal cancer/Cell proliferation/Cell migration/PI3K/AKT signaling pathway分类
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谷月,李爱芳,孙晖,李雪茹,查何,赵佳丽,谢佳卿,周兰..BCL6B对人结直肠癌LoVo细胞增殖和迁移的影响及机制[J].中国病理生理杂志,2017,33(1):38-45,8.
