中国病理生理杂志2017，Vol.33Issue(1)：67-72,6.DOI:10.3969/j.issn.1000-4718.2017.01.011
抗衰老 Klotho 蛋白对高糖作用下血管内皮细胞的保护作用
Protective effect of anti-aging Klotho protein on human umbilical vein en-dothelial cells treated with high glucose
摘要
Abstract
AIM:To study the protective effect of anti-aging Klotho protein on human umbilical vein endothe-lial cells (HUVECs) treated with high glucose (HG).METHODS:HUVECs were cultured in vitro, and divided into PBS control group, 5.5 mmol/L glucose group, 33.3 mmol/L glucose group, 0.1 μmol/L Klotho +33.3 mmol/L glucose group, 1 μmol/L Klotho+33.3 mmol/L glucose group , and 10μmol/L Klotho+33.3 mmol/L glucose group .The viabili-ty of the HUVECs was measured by MTT assay .The content of malondialdehyde ( MDA) , and the activities of lactate de-hydrogenase (LDH), superoxide dismutase (SOD) and glutathione (GSH) in cell culture supernatants were observed . The production of reactive oxygen species ( ROS) in HUVECs was analyzed by flow cytometry .The levels of nitric oxide ( NO) , endothelin ( ET-1 ) , intercellular adhesion molecule-1 ( ICAM-1 ) in HUVEC culture medium were detected by ELISA.The protein expression of nuclear factor-kappa B (NF-κB) in the HUVECs was determined by Western blot .RE-SULTS:Compared with PBS control group , 33.3 mmol/L glucose significantly decreased the HUVEC viability , increased ROS, LDH and MDA levels , reduced the activities of SOD and GSH , decreased the NO secretion , and induced the ET-1 and ICAM-1 secretion and the protein expression of NF-κB in HUVECs.When HUVECs were treated with Klotho protein at different concentrations combined with 33.3 mmol/L glucose, the cell viability was increased significantly , the ROS, LDH and MDA levels were decreased significantly , the antioxidant SOD and GSH activities were significantly increased , the se-cretion of NO was increased , but ET-1 and ICAM-1 releases and protein expression of NF-κB were significantly reduced . CONCLUSION:Anti-aging Klotho protein promotes the viability of HUVECs treated with HG , reduces the oxidative dam-age and ROS production , and restores the normal secretory function of HUVECs , thus playing a protective role in vascular endothelial cells through reducing the protein expression of NF-κB.关键词
高糖/Klotho蛋白/人脐静脉血管内皮细胞/氧化应激/动脉粥样硬化Key words
High glucose/Klotho protein/Human umbilical vein endothelial cells/Oxidative stress/Athero-sclerosis分类
医药卫生引用本文复制引用
张军,代文静,周敬群,张家俊,曹志刚..抗衰老 Klotho 蛋白对高糖作用下血管内皮细胞的保护作用[J].中国病理生理杂志,2017,33(1):67-72,6.基金项目
湖北省教育厅自然科学研究计划重点项目 （）
