中国病理生理杂志2017，Vol.33Issue(1)：166-169,173,5.DOI:10.3969/j.issn.1000-4718.2017.01.028
黄芪与葛根素联用对 KKAy 小鼠肾脏内质网应激相关 PERK 通路的影响
Effects of astragalus injection combined with puerarin injection on process of endoplasmic reticulum stress through PERK pathway in dabe-tic nephropathy mice
摘要
Abstract
AIM: To investigate the effects of astragalus injection combined with puerarin injection on endo-plasmic reticulum stress through PERK pathway in diabetic nephropathy mice .METHODS: Male KKAy mice were ran-domly divided into model group ( injected with normal saline ) and treatment group ( injected with astragalus and puerarin ) . The male C57BL/6J mice served as normal group .The mice were sacrificed 4 weeks after treatments for observing morpho-logical changes under electron microscope .The renal tissues were collected to determine the expression of protein kinase R-like endoplasmic reticulum kinase ( PERK ) , eukaryotic initiation factor 2α( eIF2α) and glucose-regulated protein 78 (GRP78) at mRNA and protein levels by real-time PCR and Western blot.RESULTS: Under electron microscope, the renal tubular epithelial cells in model group and treatment group showed the swelling of the nucleus , endoplasmic reticulum and mitochondria .The results of real-time PCR and Western blot showed that the expression of PERK , eIF2αand GRP78 at mRNA and protein levels in model group was higher than that in normal group (P<0.05), while that in treatment group was lower than that in model group .CONCLUSION: Astragalus injection combined with puerarin injection reduces the mRNA and protein expression of PERK , eIF2αand GRP78, thus inhibiting the endoplasmic reticulum stress in type 2 dia-betic mice to protect the kidney function .关键词
KKAy 小鼠/黄芪/葛根素/糖尿病肾病/内质网应激Key words
KKAy mice/Astragalus/Puerarin/Diabetic nephropathy/Endoplasmic reticulum stress分类
医药卫生引用本文复制引用
张达,李姝玉,王岩飞,李奕萱,易月娥,高誉珊,张淑静,贾德贤,王谦..黄芪与葛根素联用对 KKAy 小鼠肾脏内质网应激相关 PERK 通路的影响[J].中国病理生理杂志,2017,33(1):166-169,173,5.基金项目
国家自然科学基金资助项目（No．81072926）；北京中医药大学创新团队资助项目 （）
