中国烟草科学2016，Vol.37Issue(6)：14-20,7.DOI:10.13496/j.issn.1007-5119.2016.06.003
打顶期烟草组织基因表达分析及打顶对腋芽基因表达的影响
Analysis of Expressed Genes at the Topping Stage and Effects of Topping to Genes Expression in Axillary Buds in Nicotiana tabacum
摘要
Abstract
Topping is one of the important technical procedures of Nicotiana tabacum production. In order to study the gene expression patterns at the topping stage in Nicotiana tabacum, we selected the Daning tobacco growing area in Guangxi and collected ten tissue samples at the topping stage. The samples were used to extract total RNAs and to construct stranded specific total RNA sequencing library and to sequence using the Illumina HiSeq 2000 sequencer. Through quality control, more than 91 million clean reads were obtained and a total of 50706 genes were detected to be expressed from ten tissues at the topping stage. After normalization, screening for tissue-specific and genes differentially expressed among tested tissues, a total of 687 genes were identified to be tissue-specifical and 444 genes showed differential expression before and after topping. Above data and results will not only provide some support for exploring the molecular responses of topping, but also provide molecular targets for the regulatiion of Nicotiana tabacum vegetative and reproductive growth.关键词
烟草/打顶/转录组/生长调控Key words
Nicotiana tabacum/topping/transcriptome/growth regulation分类
农业科技引用本文复制引用
郭由兵,程廷才,李开和,卢燕回,王卫峰,夏庆友..打顶期烟草组织基因表达分析及打顶对腋芽基因表达的影响[J].中国烟草科学,2016,37(6):14-20,7.基金项目
广西壮族自治区烟草公司科技创新项目“广西贺州大宁晒烟基因组测序、表达谱芯片和代谢组学研究”(GYK201226) （GYK201226）
