中国药房2017，Vol.28Issue(1)：31-35,5.DOI:10.6039/j.issn.1001-0408.2017.01.08
安子合剂对抗磷脂抗体阳性流产小鼠TLR4/MyD88/NF-κB信号通路的影响
Effects of Anzi Mixture on TLR4/MyD88/NF-κB Signaling Pathway of Antiphospholipid Antibodies Posi-tive Abortive Mice
摘要
Abstract
OBJECTIVE:To study the effects of Anzi mixture on Toll like receptor 4(TLR4)/myeloid differentiation factor 88 (MyD88)/nuclear facter-κB(NF-κB)signaling pathway of antiphospholipid antibodies(APA)positive abortive mice,and to inves-tigate the mechanism of anti-APA positive abortion. METHODS:BALB/c mice(female)were randomly divided into blank control group,model group,aspirin group (positive control,0.0195 g/kg) and Anzi mixture low-dose,medium-dose and high-dose groups (37.7,75.4,150.8 g/kg,calculated by crude drug),with 10 mice in each group. Except for blank control group,other groups were given human β2-glycoprotein Ⅰ as derivant to establish APA positive abortion model. From the first day of pregnancy, treatment groups were given relevant medicine intragastrically,and blank control group and model group were given constant vol-ume of normal saline intragastrically,once a day,for consecutive 9 d. mRNA and protein levels of TLR4,myeloid differentiation 2 (MD2),MyD88 and NF-κB in placental tissue of mice were determined by RT-PCR and immunohistochemical method. RE-SULTS:Compared with blank control group,mRNA and protein expression of TLR4,MD2,MyD88 and NF-κB in placental tis-sue were increased markedly in the model group(P<0.01). Compared with model group,mRNA and protein expression of TLR4, MD2 and MyD88 in aspirin group and Anzi mixture low-dose and medium-dose groups were decreased significantly as well as the protein expression of TLR4 in Anzi mixture high-dose group and the protein expression of NF-κB in all medicine groups(P<0.05 or P<0.01). mRNA expression of TLR4 and MD2 and the protein expression of MD2 and MyD88 in Anzi mixture low-dose groups were lower than those in aspirin group (P<0.05 or P<0.01). CONCLUSIONS:Anzi mixture can inhibit TLR4/MyD88/NF-κB signaling pathway of APA positive abortive mice,which may be one of anti-APA positive abortion mechanisms.关键词
安子合剂/抗磷脂抗体/TLR4/MyD88/NF-κB信号通路/流产小鼠/胎盘组织Key words
Anzi mixture/Antiphospholipid antibody/TLR4/MyD88/NF-κB signaling pathway/Abortive mice/Placental tissue分类
医药卫生引用本文复制引用
柳静,陆启滨..安子合剂对抗磷脂抗体阳性流产小鼠TLR4/MyD88/NF-κB信号通路的影响[J].中国药房,2017,28(1):31-35,5.基金项目
江苏省中医药局国家中医临床研究基地开放课题(No.JD201507) （No.JD201507）
江苏省中医药局中医药领军人才课题(No. LJ200910) （No. LJ200910）
江苏省中医院2014年度院级课题博士项目(No.Y14019) （No.Y14019）
