中国药房2017，Vol.28Issue(1)：67-71,5.DOI:10.6039/j.issn.1001-0408.2017.01.18
黎药枫蓼肠胃康颗粒对幼龄大/小鼠的抗炎、镇痛、保护胃黏膜及解痉作用研究
Study on Anti-inflammatory,Analgesic,Gastric Mucosa-protective and Spasmolysis Effects of Li Medicine Fengliao Changweikang Granule
摘要
Abstract
OBJECTIVE:To investigate anti-inflammatory,analgesic,gastric mucosa-protective and spasmolysis effects of Li medicine Fengliao changweikang granule(FMCWK)in juvenile rats/mice. METHODS:60 SD juvenile rats were randomly divided into blank group (distilled water),Baohe pills group (TCM positive control,0.98 g/kg,calculated by crude drug),omeprazole group (chemicals positive control,4.36 × 10-3 g/kg) and FMCWK low-dose,medium-dose and high-dose groups (4.88,9.75, 19.50 g/kg,calculated by crude drug),with 10 rats in each group. They were given relevant medicine intragastrically,once a day, for consecutive 7 d. The secretion of gastric juice,pH value,total acidity,1 h total acid output and the activity of pepsase were all determined. 70 SD juvenile rats were grouped with same method,and gastric mucosal lesion model group(distilled water)was al-so set up. They were given relevant medicine intragastrically,once a day,for consecutive 4 d. The index of gastric mucosal lesion was determined. 60 KM juvenile mice were randomly divided into blank group(distilled water),indometacin group(positive con-trol,9.79 × 10-3 g/kg) and FMWK low-dose,medium-dose and high-dose groups (4.88,9.75,19.50 g/kg,calculated by crude drug),with 12 mice in each group. They were given relevant medicine intragastrically,once a day,for consecutive 4 d. The perito-neal capillary permeability test was adopted to determine the content of Evans blue solution in mice;acetic acid induced pain meth-od was used to determine the number of writhing within 15 min;anti-inflammatory and analgesic effects of FMCWK were investi-gated. The gastric fundus strips with the length of 2 cm was collected to induce spasm by BaCl2. The effects of FMCWK(29,87, 294 g/L,calculated by crude drug)and Baohe pills(22 g/L,calculated by crude drug)on gastric fundus strips tension were investi-gated and the spasmolysis percentage was also calculated to investigate its spasmolysis effect. RESULTS:Compared with blank group,high-dose FMCWK significantly increased pH of gastric juice,and decreased total acidity,1 h total acid output of rats,de-creased the content of Evans blue and the number of writhing within 15 min in mice (P<0.05 or P<0.01);different concentra-tions of FMCWK significantly decreased gastric fundus strips tension while decreased spasmolysis percentage of rats (P<0.01). Compared with model group,medium-dose and high-dose FMCWK significantly decreased the index of gastric mucosal lesion in rats (P<0.05 or P<0.01). CONCLUSIONS:FMCWK possesses certain anti-inflammatory,analgesic,gastric mucosa-protective and spasmolysis effects for juvenile rats/mice.关键词
黎药/枫蓼肠胃康颗粒/幼龄大鼠/幼龄小鼠/胃黏膜损伤/抗炎/镇痛/解痉Key words
Li medicine/Fengliao changweikang granule/Juvenile rats/Juvenile mice/Gastric mucosal lesion/Anti-inflam-matory/Analgesic/Spasmolysis分类
医药卫生引用本文复制引用
茹丽,刘静,张志兰,沙莹,袁征,刘婵,李芷君,邢成锋,关业枝..黎药枫蓼肠胃康颗粒对幼龄大/小鼠的抗炎、镇痛、保护胃黏膜及解痉作用研究[J].中国药房,2017,28(1):67-71,5.基金项目
广东省自然科学基金资助项目(No.2016A030313646) （No.2016A030313646）
广东省科技计划项目(No.2015A040404042) （No.2015A040404042）
