中国药房2017，Vol.28Issue(1)：122-126,5.DOI:10.6039/j.issn.1001-0408.2017.01.32
18家企业阿胶中氨基酸的含量分析与比较研究
Content Analysis and Comparative Study of Amino Acids in Ejiao from 18 Manufacturers
摘要
Abstract
OBJECTIVE:To provide reference for quality evaluation of Ejiao. METHODS:The contents of 17 amino acids in 18 batches of Ejiao from 18 manufacturers were analyzed by automatic amino acid analyzer;the content data was processed by prin-cipal component analysis (PCA) and hierarchical clustering analysis (HCA),in order to understand the relationship among these amino acids and categorize the Ejiao products. RESULTS:Except for a batch of Ejiao,the other 17 batches contained 17 various amino acids,including 7 necessary amino acids(Thr,Val,Met,Ile,Leu,Phe and Lys)for human being,especially the contents of Gly and Pro were higher. The total content of amino acids was arranged from 66.1% to 82.0%,showing great difference. Four main components were extracted by PCA,the cumulative contribution of which was 89.578%,and accordingly it may be consid-ered that Pro,Ala,Glu,Asp,Leu,Ile and Thr were characteristic amino acids of Ejiao products. In hierarchical clustering analy-sis,3 tree figures of commercial Ejiao product were achieved and Euclidean distance was varied among 0-25. CONCLUSIONS:There is great difference in total control of amino acid in commercial Ejiao product;Ejiao products from various manufacturers dif-fer in their amino acid contents. Ejiao is rich in collagen,and therefore,using amino acid determination would be helpful to moni-tor its quality.关键词
阿胶/氨基酸/主成分分析/聚类分析Key words
Ejiao/Amino acids/Principal component analysis/Hierarchical clustering analysis分类
医药卫生引用本文复制引用
葛重宇,庞慧,李楠,郑洁,何昌隆,杨欢,沈玉萍..18家企业阿胶中氨基酸的含量分析与比较研究[J].中国药房,2017,28(1):122-126,5.基金项目
国家自然科学基金资助项目(No.81303174) （No.81303174）
江苏省科技项目(No.BK20150463) （No.BK20150463）
中国博士后科学基金面上资助项目(No.2016M590425) （No.2016M590425）
江苏大学大学生科研立项项目(No.14A457) （No.14A457）
