中国中医药信息杂志2017，Vol.24Issue(2)：60-64,5.DOI:10.3969/j.issn.1005-5304.2017.02.016
桦木酸对人肝癌HepG2肿瘤干细胞增殖的影响
Effects of Betulinic Acid on Proliferation of Human Liver Cancer HepG2 Cells
摘要
Abstract
Objective To observe the effects of betulinic acid (BA) on proliferation of human hepatoma stem cell;To discuss its anti-cancer mechanism from the aspects of cell cycle and cell apoptosis. Methods HepG2 stem cells were cultivated in vitro and testified the self-renewal capacity. The effects of BA in concentration of 40, 20, 10, 5, 2.5, 1.25μmol/L on the cell vitality of cultured human liver cancer stem cells for 24 and 48 hours were measured with CCK-8 method. The human hepatoma stem cell line HepG2 was administrated by BA at concentrations of 40, 20, 10, 5μmol/L for 48 hours, and cell cycle and apoptosis rate were measured by flow cytometry. Results BA could inhibit HepG2 stem cell proliferation obviously with dose-effect relationship. BA influenced cell cycle, and induced tumor stem cell apoptosis. 40μmol/L BA blocked cell cycle in S phase, and cell apoptosis rate reached 10.86%. Conclusion BA has obvious inhibitory effects on proliferation of HepG2 liver cancer stem cell, which probably plays a part in anti-cancer by influencing cell cycle and inducing cell apoptosis.关键词
桦木酸/石见穿/人肝癌HepG2细胞/肿瘤干细胞/细胞凋亡Key words
betulinic acid/Salvia chinensis/human hepatoma cells/cancer stem cell/cell apoptosis分类
医药卫生引用本文复制引用
陈飞宇,李澎,甘加宽,张安娜,任钧国,刘建勋..桦木酸对人肝癌HepG2肿瘤干细胞增殖的影响[J].中国中医药信息杂志,2017,24(2):60-64,5.基金项目
中国中医科学院自主选题项目 （）
