中华耳科学杂志2016，Vol.14Issue(6)：735-739,5.DOI:10.3969/j.issn.1672-2922.2016.06.008
小型猪听性脑干反应分析及脉冲噪声暴露后的变化
Characteristics of auditory brainstem responses in miniature pigs and changes after impulse noise exposure
摘要
Abstract
Objective To investigate the characteristics of auditory brainstem responses (ABRs) in healthy miniature pigs and their changes after impulse noise exposure. Methods Healthy miniature pigs were exposed to impulse noise at 30, 50, 80 or 100 pulses separately, and auditory brainstem responses were tested before and immediately, at 1, 2, 4 and 8 weeks after exposure. Auditory brainstem response characteristics and changes after noise exposure were recorded. Results ABR waves in healthy miniature pigs showed five typical peaks, consistent with those in other species. The mean threshold of click-ABRs was 25±5 dB SPL. After high intensity impulse noise exposure, the ABR threshold was significantly increased. No identifiable ABR waves could be detected immediately after exposure to more than 50 pulses at the maximum system output. Tone burst ABR thresholds at 4, 8, 16, 20 and 32 kHz also increased to higher than 90 dB SPL immediately after noise exposure. At 1 week after exposure, various degrees of thresholds recovery were observed, constituting most recovery after noise exposure. From the second to the eighth week, threshold recovery was plateaued. Recovery of tone burst ABR thresholds at 4, 8, 16, 20 and 32 kHz were consistent with that of click ABR, with no statistically significant differences among the frequencies. Furthermore, ABR waves showed poor differentiation as the time went on, despite recovery in threshold. Conclusions ABR waves in miniature pigs are similar to those of rodents and humans, but are more sensitive to impulsive noise exposure than in rodents, albeit with shorter recovery period. In addition, because of the high similarities in genetics, anatomy and pathophysiology between miniature pigs and humans, miniature pigs are an ideal large mammal mod-el for auditory research.关键词
小型猪/听性脑干反应/脉冲噪声Key words
Miniature pig/auditory brainstem response/impulse noise分类
医药卫生引用本文复制引用
陈志婷,王方园,冀飞,杨仕明,吴南..小型猪听性脑干反应分析及脉冲噪声暴露后的变化[J].中华耳科学杂志,2016,14(6):735-739,5.基金项目
国家863青年科学家项目(2014AA020510)；国家973计划重大科学研究计划干细胞项目（2012CB967900)；国家科技部新药创制重大专项（2014ZX09J14101-06C）；国家自然科学基金项目 （2014AA020510）
中国科协创新驱动助力工程（2016CXQD01）Funds by The National High-tech R$D Program of China for young Scientists (2014AA020510), National Basic Research Program of China (973 Program)(#2012CB967900), National Ministry of science and technology new drug creation major project (2014ZX09J14101-06C) （2014AA020510）
National Natural Science Foundation of China (NSFC 81470700)；Innovation and drive power engineering proj-ect of China association for science and technology(2016CXQD01) （NSFC 81470700）
