中华耳科学杂志2016，Vol.14Issue(6)：788-792,5.DOI:10.3969/j.issn.1672-2922.2016.06.017
醛固酮对豚鼠耳蜗功能及AF9蛋白表达的调节作用
Regulation of AF9 protein expression and cochlear function by aldosterone in guinea pigs
摘要
Abstract
Objective To study the effect of aldosterone on AF9 protein expression and cochlear function in guinea pigs and to investigate possible roles of aldosterone and AF9 protein in the pathogenesis of endolymphatic hydrops. Meth-ods Twenty-eight guinea pigs were randomly divided into a control and an aldosterone treatment group (Ald group). The Ald group received daily aldosterone at 0.1mg/kg/d intraperitoneally for 5 days, while the control group received equivalent normal saline treatment. After one month, cochlear function was tested by auditory brainstem response (ABR), morphology of cochlea was examined by Hematoxylin and Eosin staining, and expression of AF9 protein was evaluated by immunohisto-chemistry. Rusults Compared to the control group, the Ald group showed higher ABR thresholds (P<0.05). Seventy-one percent of animals in the Ald group had endolymphatic hydrops. The AF9 protein was found in the stria vascularis, Reiss-ner’s membrane, spiral limbus, organ of Corti, spiral ganglions and periosteum of scala vestibule. The expression of AF9 protein in stria vascularis and Reissner’s membrane in the Ald group was lower than that in the control group (P<0.05). Conclusion AF9 protein involved in Na+regulation is present in the cochlea. Aldosterone participates in endolymph metab-olism and influence the function of inner ear by regulating the expression of AF9 protein.关键词
醛固酮/AF9蛋白/内淋巴积水/耳蜗功能Key words
Aldosterone/AF9 protein/Endolymphatic hydrops/Cochlear function分类
医药卫生引用本文复制引用
秦丽,章碧云,钟时勋..醛固酮对豚鼠耳蜗功能及AF9蛋白表达的调节作用[J].中华耳科学杂志,2016,14(6):788-792,5.基金项目
1.重庆市自然科学基金项目资助（cstc2013jcy-jA10034）2.国家临床重点专科建设经费项目卫办医政函[2012]649号Fund projects：Chongqing Natural Science Foundation (cstc2013jcyjA10034) （cstc2013jcyjA10034）
National Key Clinical Specialties Construction Pro-gram of China([2012]649) （[2012]649）
