中华耳科学杂志2016，Vol.14Issue(6)：793-797,5.DOI:10.3969/j.issn.1672-2922.2016.06.018
CMC-TAT蛋白转导肽纳米载体体外转染效果评估
Transduction efficiency and cytotoxicity evaluation of CMC-TAT nanocarriers in vitro
摘要
Abstract
Objective To construct O-carboxymethy chitosan-TAT nanoparticles(CTNs), and to explore the efficiency and security of CTNs for miR96 by transfect 293 cells and cochlear basilar membrane. Method The CTNs were generated with the optimal weight ratio of 1.5:1 (CMC:TAT), which were used for the incorporation with miR-96 mimisc marked cy3. Transfect 293 cells and cochlear basilar membrane with Lipofectamine-miR96-cy3 and CTNs-miR96-cy3 at the concentra-tion of 150nM with control. The red fluorescence positive cells were counted to evaluate the efficiency of CTNs. The securi-ty of CTNs was evaluated by MTT assay. Result CTNs with a size of 186.8 nm and a zeta potential of-30.9 mV were suc-cessfully prepared. The transduction efficiency of CTNs-miR96-cy3 was lower than that of Lipofectamine-miR96-cy3 in both 293 cells and cochlear basilar membrane. MTT assay showed that the cytotoxicity of CTNs was significantly lower than that of Lipofectamine. Conclusion CTNs can successfully transduct miR96 in vitro, which reveals the feasibility and se-curity of CTNs as delivery vector for miRNA in cochlea,however, it’s transduction efficiency is low.关键词
羧甲基壳聚糖-TAT蛋白转导肽纳米载体/miRNA/miR96/内耳转染Key words
O-carboxymethy chitosan-TAT Nanocarriers/miRNA/miR96/Transfection in cochlea分类
医药卫生引用本文复制引用
唐朝颖,韩维举,邓雄威,王方园,袁硕龙,杨仕明,吴南..CMC-TAT蛋白转导肽纳米载体体外转染效果评估[J].中华耳科学杂志,2016,14(6):793-797,5.基金项目
国家863青年科学家项目（2014AA020510），国家自然科学基金面上项目（81470683），中国科协创新驱动助力工程（2016CX-QD01）,国家973计划重大科学研究计划干细胞项目（2012CB967900,其子课题:2012CB967901,2012CB967903）。National High-tech R&D Program of China for Young Scientists (2014AA020510), National Natural Science Foundation of China (81470683),Innovation and drive power engineering project of China association for science and technology（2016CXQD01），National Ba-sic Research Program of China (973 Program)(2012CB967900, It's Branch programs 2012CB967901,2012CB967903) （2014AA020510）
