中山大学学报（自然科学版）2017，Vol.56Issue(1)：121-124,4.DOI:10.13471/j.cnki.acta.snus.2017.01.019
白背飞虱18S核糖体基因克隆及系统发育
Clonging and phylogenetic analysis of 1 8 S rDNA gene from Sogatella furcifera (Horvath)
摘要
Abstract
The long wing female adults of Sogatella furcifera were selected to extract their genomic DNA. According to the complete 1 8S rDNA gene sequence from Hemiptera,the unknown 1 8S rDNA sequence of S.furcifera was amplified by PCR.The complete 1 8S rDNA gene sequence of S.furcifera was obtained for the first time by splicing the unknown and known 1 8S rDNA sequences,whole sequence length was 1 891 bp.Comparing 1 8S rDNA sequence from S.furcifera and those from other insects,four conserved re-gions were found and the second region had the least insertion or deletion.Based on the second region, phylogenetic analysis was made and the results showed that most Homoptera insects used in this study had a close relatives with Eurydema maracandica (Hemiptera)except Delphacida.关键词
核糖体/1 8S rDNA/白背飞虱Key words
ribosome/18S rDNA/Sogatella furcifera分类
生物科学引用本文复制引用
梁梓强,梁士可,刘婷婷,李广宏,王方海..白背飞虱18S核糖体基因克隆及系统发育[J].中山大学学报（自然科学版）,2017,56(1):121-124,4.基金项目
广东省自然科学基金（S2022020002353，031628）；国家自然科学基金 （）
