作物研究2017，Vol.31Issue(1)：11-14,24,5.DOI:10.16848/j.cnki.issn.1001-5280.2017.01.03
MAS 育种改良籼稻恢复系‘E32’及其杂交种的稻瘟病抗性
I mproving BIast Resistance of Rice Restorer ‘E32’and Its Hybrid through MoIecuIar Marker -assisted SeIection
摘要
Abstract
To improve blast resistance of the indica rice restorer E32 and its hybrid,a molecular marker,Ins2 -3,was de-veloped based on the DNA sequence of the cloned broad -spectrum blast resistance gene Pi9,and marker -assisted selec-tion breeding practice was implemented in this study.The main results were as follows:Ins2 -3 was a co -dominant mark-er,showing stable polymorphism between the Pi9 donor 75 -1 -127 and the receptor E32 by producing a 793bp and a 2kb PCR band respectively,and the phenotype selection efficiency by marker genotype reached 100%.A new restorer line E32-Pi9 and its hybrid Peiai64s/E32 -Pi9,with high -level seedling and spike blast resistance and unchanged genetic background,were bred.关键词
水稻/稻瘟病抗性改良/Pi9 基因/分子标记辅助选择Key words
rice/blast resistance improvement/the Pi9 gene/molecular marker -assisted selection分类
农业科技引用本文复制引用
邹俊锋,李永聪,刘雄伦,刘金灵,陈海龙,杨丰宇,黄俊,廖花..MAS 育种改良籼稻恢复系‘E32’及其杂交种的稻瘟病抗性[J].作物研究,2017,31(1):11-14,24,5.基金项目
国家转基因生物新品种培育重大专项（2016ZX08001-002）；湖南省科技重大专项（2015NK1001）。 （）
