作物学报2017，Vol.43Issue(1)：1-8,8.DOI:10.3724/SP.J.1006.2017.00001
菜豆普通细菌性疫病抗性基因定位
Mapping of Common Bacterial Blight Resistance Gene in Common Bean
摘要
Abstract
Common bacterial blight disease is a serious disease affecting the production of common bean (Phaseolus vulgaris L.) worldwide. The common bean germplasm Longyundou 5 is the main cultivar carrying common bacterial blight resistance gene in Heilongjiang province. To study the genetic mechanisms behind this resistance, we constructed 785 F2 plants from Longyundou 5. Linkage analysis was performed on this population by using SSR markers. A linkage map covering 1648.42 cM with an average marker distance of 8.00 cM among 206 SSR markers was constructed. This map contained 12 linkage groups with a mean group length of 137.37 cM and the number of loci ranging from three to thirty-five. Combined with the results of phenotypic evaluation, QTL analysis was performed by the inclusive composite interval mapping method with QTL IciMapping v4.0. A QTL was de-tected betweenp6s249 andp6s183 on chromosome Pv06, with an additive effect of 0.44, which means the favourable gene of this locus is from Longyundou 5. The LOD score of the QTL was 5.93 and the total phenotypic variation at 14 days after inoculation was 4.61%. These results showed the effect of the QTL was low, which will play an important role in breeding durable resistant varieties of common bean. Association analysis between 11 SSR primers linked with common bacterial blight resistance and the disease rating showed that SSR markerp6s249 is excellently associated (P<0.001) with common bacterial blight resistance and can be used for marker assisted selection.关键词
普通菜豆/普通细菌性疫病/SSR/QTL/关联分析Key words
Phaseolus vulgaris L/Common bacterial blight/SSR/QTL/Association analysis引用本文复制引用
朱吉风,武晶,王兰芬,朱振东,王述民..菜豆普通细菌性疫病抗性基因定位[J].作物学报,2017,43(1):1-8,8.基金项目
本研究由国家现代农业产业技术体系建设专项(CARS-09),国家科技支撑计划项目(2013BAD01B03-18a)和中国农业科学院科技创新工程项目资助。 This study was supported by the China Agriculture Research System (CARS-09), the National Key Technology R&D Program of China (2013BAD01B03-18a), and the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences （CARS-09）
